使用径向密度图校准冷冻水合标本的图像放大倍数。
Use of radial density plots to calibrate image magnification for frozen-hydrated specimens.
作者信息
Belnap D M, Grochulski W D, Olson N H, Baker T S
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
出版信息
Ultramicroscopy. 1993 Mar;48(3):347-58. doi: 10.1016/0304-3991(93)90110-j.
Abstract
Accurate magnification calibration for transmission electron microscopy is best achieved with the use of appropriate standards and an objective calibration technique. We have developed a reliable method for calibrating the magnification of images from frozen-hydrated specimens. Invariant features in radial density plots of a standard are compared with the corresponding features in a "defocused" X-ray model of the same standard. Defocused X-ray models were generated to mimic the conditions of cryo-electron microscopy. The technique is demonstrated with polyoma virus, which was used as an internal standard to calibrate micrographs of bovine papilloma virus type 1 and bacteriophage phi X174. Calibrations of the micrographs were estimated to be accurate to 0.35%-0.5%. Accurate scaling of a three-dimensional structure allows additional calibrations to be made with radial density plots computed from two- or three-dimensional data.
摘要
透射电子显微镜的精确放大倍数校准最好通过使用适当的标准物和客观校准技术来实现。我们已经开发出一种可靠的方法来校准来自冷冻水合标本图像的放大倍数。将标准物径向密度图中的不变特征与同一标准物的“散焦”X射线模型中的相应特征进行比较。生成散焦X射线模型以模拟冷冻电子显微镜的条件。该技术通过多瘤病毒进行了演示，多瘤病毒被用作内标来校准1型牛乳头瘤病毒和噬菌体φX174的显微照片。显微照片的校准估计精确到0.35%-0.5%。三维结构的精确缩放允许使用从二维或三维数据计算出的径向密度图进行额外的校准。
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本文引用的文献
1
Radial distributions of density within macromolecular complexes determined from dark-field electron micrographs.由暗场电子显微照片确定的大分子复合物内密度的径向分布。
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6363-7. doi: 10.1073/pnas.81.20.6363.
2
Cryo-electron microscopy of viruses.病毒的冷冻电子显微镜技术
Nature. 1984;308(5954):32-6. doi: 10.1038/308032a0.
3
Polyoma virus capsid structure at 22.5 A resolution.多瘤病毒衣壳结构，分辨率为22.5埃。
Nature. 1982 Jan 14;295(5845):110-5. doi: 10.1038/295110a0.
4
The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy.作为电子显微镜长度内标的结晶过氧化氢酶的晶格间距。
J Ultrastruct Res. 1968 Sep;24(5):454-64. doi: 10.1016/s0022-5320(68)80048-6.
5
Electron microscopy of frozen-hydrated biological material.冷冻水合生物材料的电子显微镜检查。
Nature. 1986;319(6055):631-6. doi: 10.1038/319631a0.
6
The T=4 envelope of Sindbis virus is organized by interactions with a complementary T=3 capsid.辛德毕斯病毒的T=4包膜是通过与互补的T=3衣壳相互作用而形成的。
Cell. 1987 Mar 27;48(6):923-34. doi: 10.1016/0092-8674(87)90701-x.
7
Contrast transfer for frozen-hydrated specimens: determination from pairs of defocused images.冷冻水合标本的对比度传递：通过成对散焦图像进行测定
Ultramicroscopy. 1988;25(4):279-91. doi: 10.1016/0304-3991(88)90003-4.
8
Electron microscopy of frozen, hydrated biological specimens.冷冻水合生物标本的电子显微镜检查。
Annu Rev Biophys Biophys Chem. 1986;15:237-57. doi: 10.1146/annurev.bb.15.060186.001321.
9
Cryo-electron microscopy of vitrified specimens.玻璃化标本的冷冻电子显微镜检查。
Q Rev Biophys. 1988 May;21(2):129-228. doi: 10.1017/s0033583500004297.
10
Reconstruction of the three-dimensional structure of simian virus 40 and visualization of the chromatin core.猿猴病毒40三维结构的重建及染色质核心的可视化
Proc Natl Acad Sci U S A. 1988 Jan;85(2):422-6. doi: 10.1073/pnas.85.2.422.
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