Abdelgawad Abdelrahman M, El-Naggar Mehrez E, Hudson Samuel M, Rojas Orlando J
Biobased Colloids and Materials Group (BiCMat), Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University, Campus Box 8005, NC, USA; Textile Research Division, National Research Center (Affiliation ID:60014618), Dokki, Cairo, Egypt.
Biobased Colloids and Materials Group (BiCMat), Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University, Campus Box 8005, NC, USA; Textile Research Division, National Research Center (Affiliation ID:60014618), Dokki, Cairo, Egypt.
Int J Biol Macromol. 2017 Jan;94(Pt A):96-105. doi: 10.1016/j.ijbiomac.2016.07.061. Epub 2016 Sep 30.
Two chitosan derivatives, namely, thiol-chitosan (TCs) and chitosan iodoacetamide (CsIA) were newly synthesized by reacting Cs with thiglycolic acid (TGA) and iodoacetic acid (IA) respectively. After being crosslinked with glutraldehyde (GA), the two derivatives were submitted to FT-IR and H NMR analysis for identification and characterization of their chemical features. As TCs and CsIA are water soluble, their electrospun nanofibres mats from aqueous solutions could be crosslinked and achieved using polyvinyl alcohol/Chitosan blend (PVA/Cs) polymers. Morphological structures of the obtained nanofibres and their webs were studied via those of TCs and CsIA free systems. The data also indicate that the crosslinked PVA/Cs/CsIA is more thermally stable than the crosslinked PVA/Cs/TCs and crosslinked PVA/Cs respectively. It was proved that the electrospun fibers containing TCs or CsIA display a superior antibacterial activity against negative bacteria E. Coli with a minimum inhibitory concentration (MIC) of 400μg/ml. These effects are rather in confirmation with bacterial kinetics essays which were also carried out in current work. Of particular interest is that the antimicrobial properties of fibers containing small concentration of either TCs or CsIA are much superior than those obtained with neat Cs electrospun nanofibres used as reference. By and large the results advocate the fibers webs containing TCs or CsIA as excellent candidates for wound dressing applications.
两种壳聚糖衍生物，即硫醇化壳聚糖（TCs）和壳聚糖碘乙酰胺（CsIA），分别通过壳聚糖（Cs）与巯基乙酸（TGA）和碘乙酸（IA）反应新合成。与戊二醛（GA）交联后，对这两种衍生物进行傅里叶变换红外光谱（FT-IR）和核磁共振氢谱（H NMR）分析，以鉴定和表征其化学特征。由于TCs和CsIA是水溶性的，它们从水溶液中静电纺丝得到的纳米纤维毡可以使用聚乙烯醇/壳聚糖共混物（PVA/Cs）聚合物进行交联并制成。通过不含TCs和CsIA的体系研究了所得纳米纤维及其纤维网的形态结构。数据还表明，交联的PVA/Cs/CsIA分别比交联的PVA/Cs/TCs和交联的PVA/Cs具有更高的热稳定性。事实证明，含有TCs或CsIA的静电纺丝纤维对阴性细菌大肠杆菌具有优异的抗菌活性，最低抑菌浓度（MIC）为400μg/ml。这些结果与本研究中进行的细菌动力学试验结果相当一致。特别值得注意的是，含有低浓度TCs或CsIA的纤维的抗菌性能比用作对照的纯壳聚糖静电纺纳米纤维的抗菌性能优越得多。总体而言，结果表明含有TCs或CsIA的纤维网是伤口敷料应用的极佳候选材料。
