Liao Ying, Brandt Bernd W, Zhang Min, Li Jiyao, Crielaard Wim, van Loveren Cor, Deng Dong Mei
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China.
Sichuan University West China College of Stomatology, Chengdu, Sichuan, China.
Antimicrob Agents Chemother. 2016 Nov 21;60(12):7509-7512. doi: 10.1128/AAC.01366-16. Print 2016 Dec.
Previously, we identified a single nucleotide mutation in the promoter (mutp) of the fluoride antiporter-coding genes in a naturally fluoride-resistant Streptococcus mutans strain. Here, we studied the role of this mutation in a defined genetic background. The results confirmed that this mutation alone confers fluoride resistance on S. mutans, as shown by growth and lactic acid production assays. This resistance was explained by constitutively higher mutp promoter activity and upregulation of the fluoride antiporter-coding genes.
此前，我们在一株天然耐氟的变形链球菌中鉴定出氟离子反向转运体编码基因启动子中的一个单核苷酸突变（mutp）。在此，我们在明确的遗传背景下研究了该突变的作用。生长和乳酸产生试验结果证实，仅这一突变就赋予了变形链球菌氟抗性。这种抗性可通过持续较高的mutp启动子活性以及氟离子反向转运体编码基因的上调来解释。
