Singh Anamika, Patani Anil, Patel Margi, Vyas Suhas, Verma Rakesh Kumar, Amari Abdelfattah, Osman Haitham, Rathod Lokendra, Elboughdiri Noureddine, Yadav Virendra Kumar, Sahoo Dipak Kumar, Chundawat Rajendra Singh, Patel Ashish
School of Liberal Arts and Sciences, Mody University of Science and Technology, Sikar, India.
Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College, Sankalchand Patel University, Visnagar, India.
Front Microbiol. 2024 Jan 4;14:1330071. doi: 10.3389/fmicb.2023.1330071. eCollection 2023.
The primary goal of this experiment is to examine the effectiveness of strain PAR as a rhizobacterium that promotes plant growth in mitigating the negative effects of fluoride-induced stress in tomato ( Mill.) plants. A total of 16 rhizobacterial strains were tested for plant growth-promoting (PGP) attributes, with isolates S1, S2, and S3 exhibiting different characteristics. Furthermore, growth kinetics studies revealed that these isolates were resilient to fluoride stress (10, 20, 40, and 80 ppm), with isolate S2 exhibiting notable resilience compared to the other two strains. Phylogenetic analysis revealed isolate S2 as strain PAR. Physiological analyses demonstrated that strain PAR had a beneficial impact on plant properties under fluoride stress, comprising seed germination, root length, shoot height, relative water content, and leaf area, the strain also impacted the buildup of glycine betaine, soluble sugar, and proline, demonstrating its significance in enhancing plant stress tolerance. In strain PAR-treated plants, chlorophyll content increased while malondialdehyde (MDA) levels decreased, indicating enhanced photosynthetic efficiency and less oxidative stress. The strain modified antioxidant enzyme action (catalase, ascorbate, glutathione reductase, peroxidase, and superoxide dismutase), which contributed to improved stress resilience. Mineral analysis revealed a decrease in sodium and fluoride concentrations while increasing magnesium, potassium, phosphorus, and iron levels, emphasizing the strain's significance in nutrient management. Correlation and principal component analysis revealed extensive correlations between physiological and biochemical parameters, underscoring strain PAR's multifaceted impact on plant growth and stress response. This study offers valuable information on effectively utilizing PGPR, particularly strain PAR, in fluoride-contaminated soils for sustainable agriculture. It presents a promising biological strategy to enhance crop resilience and productivity.
本实验的主要目标是研究根际细菌PAR菌株作为一种促进植物生长的根际细菌，在减轻氟诱导的胁迫对番茄（Mill.）植株负面影响方面的有效性。共测试了16种根际细菌菌株的植物生长促进（PGP）特性，分离株S1、S2和S3表现出不同的特征。此外，生长动力学研究表明，这些分离株对氟胁迫（10、20、40和80 ppm）具有抗性，与其他两种菌株相比，分离株S2表现出显著的抗性。系统发育分析表明分离株S2为PAR菌株。生理分析表明，PAR菌株在氟胁迫下对植物特性具有有益影响，包括种子萌发、根长、茎高、相对含水量和叶面积，该菌株还影响了甘氨酸甜菜碱、可溶性糖和脯氨酸的积累，表明其在增强植物胁迫耐受性方面的重要性。在经PAR菌株处理的植株中，叶绿素含量增加而丙二醛（MDA）水平降低，表明光合效率提高且氧化应激减轻。该菌株改变了抗氧化酶的活性（过氧化氢酶、抗坏血酸、谷胱甘肽还原酶、过氧化物酶和超氧化物歧化酶），这有助于提高胁迫抗性。矿物质分析表明钠和氟浓度降低，而镁、钾、磷和铁水平升高，强调了该菌株在养分管理方面的重要性。相关性和主成分分析表明生理和生化参数之间存在广泛的相关性，突出了PAR菌株对植物生长和胁迫反应的多方面影响。本研究为在氟污染土壤中有效利用植物根际促生菌（PGPR），特别是PAR菌株以实现可持续农业提供了有价值的信息。它提出了一种增强作物抗性和生产力的有前景的生物学策略。
