Wang Yuhua, Xue Xueyi, Zhu Jian-Kang, Dong Juan
Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Development. 2016 Dec 1;143(23):4452-4461. doi: 10.1242/dev.129932. Epub 2016 Oct 3.
DNA methylation and histone modifications interact to modulate gene expression in biological organisms. The histone demethylase IBM1 suppresses DNA methylation and gene silencing, primarily by targeting genic regions in the Arabidopsis genome. The chromatin regulator EDM2 is also required for prevention of genic DNA methylation because it maintains IBM1 expression by promoting IBM1 mRNA distal polyadenylation. Loss-of-function ibm1 and edm2 mutant plants display a wide range of developmental defects, but little is known about which developmentally important genes are regulated by IBM1 and EDM2. Here, we show that both ibm1 and edm2 mutants display defects in production of stomatal lineage cells, which is linked to DNA hypermethylation of the ERECTA family genes, including ER, ERL1 and ERL2 Stomatal phenotypes and DNA methylation levels of ER genes in ibm1 and edm2 mutants are restored by mutations in the genes encoding the histone methyltransferase KYP and DNA methyltransferase CMT3. Our data demonstrate that a specific plant developmental context is influenced by IBM1-regulated histone modification and DNA methylation on the gene body region of the ERECTA receptors.
DNA甲基化和组蛋白修饰相互作用，以调节生物体内的基因表达。组蛋白去甲基化酶IBM1主要通过靶向拟南芥基因组中的基因区域来抑制DNA甲基化和基因沉默。染色质调节因子EDM2对于防止基因DNA甲基化也是必需的，因为它通过促进IBM1 mRNA远端多聚腺苷酸化来维持IBM1的表达。功能缺失的ibm1和edm2突变体植物表现出广泛的发育缺陷，但对于哪些发育重要基因受IBM1和EDM2调控却知之甚少。在这里，我们表明ibm1和edm2突变体在气孔谱系细胞的产生上均表现出缺陷，这与ERECTA家族基因（包括ER、ERL1和ERL2）的DNA高甲基化有关。编码组蛋白甲基转移酶KYP和DNA甲基转移酶CMT3的基因突变可恢复ibm1和edm2突变体中ER基因的气孔表型和DNA甲基化水平。我们的数据表明，特定的植物发育背景受IBM1调节的组蛋白修饰和ERECTA受体基因体区域上的DNA甲基化影响。
