Paul Corry, Rho HeeSool, Neiswinger Johnathan, Zhu Heng
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
Cold Spring Harb Protoc. 2016 Oct 3;2016(10):2016/10/pdb.prot087965. doi: 10.1101/pdb.prot087965.
Functional protein microarrays allow fast, straightforward, and efficient high-throughput screening of protein-protein interactions. The microarray approach has outpaced other interaction methods, such as yeast two-hybrid screens, in part because of the vast amounts of information that can be obtained during a single assay. This protocol describes how to perform a binding assay for a protein of interest using a proteome microarray composed of thousands of functional, recombinant proteins adhered to a microchip.
功能蛋白质微阵列可实现对蛋白质-蛋白质相互作用的快速、直接且高效的高通量筛选。微阵列方法已超越其他相互作用研究方法，如酵母双杂交筛选，部分原因在于单次检测可获取海量信息。本方案介绍了如何使用由附着于微芯片上的数千种功能性重组蛋白组成的蛋白质组微阵列，对感兴趣的蛋白质进行结合检测。
