Lu Kuan-Yi, Chen Chien-Sheng, Neiswinger Johnathan, Zhu Heng
Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cold Spring Harb Protoc. 2016 Oct 3;2016(10):2016/10/pdb.prot087981. doi: 10.1101/pdb.prot087981.
Studying lipid-protein interactions is central to understanding lipid signaling, a key regulatory system in cells. To better identify lipid-binding proteins, we developed a nonquenched fluorescent (NQF) liposome that is able to carry both fluorescent molecules and a lipid of interest. By combining the strength of NQF liposomes with protein microarray technology, the method presented here facilitates high-throughput screening of lipid-protein interactions. This protocol describes how to prepare NQF liposomes and apply the fabricated liposomes to yeast proteome microarrays.
研究脂质-蛋白质相互作用是理解脂质信号传导的核心，而脂质信号传导是细胞中的关键调节系统。为了更好地识别脂质结合蛋白，我们开发了一种非淬灭荧光（NQF）脂质体，它能够携带荧光分子和感兴趣的脂质。通过将NQF脂质体的优势与蛋白质微阵列技术相结合，本文介绍的方法有助于高通量筛选脂质-蛋白质相互作用。本方案描述了如何制备NQF脂质体，并将制备好的脂质体应用于酵母蛋白质组微阵列。
