Neiswinger Johnathan, Uzoma Ijeoma, Cox Eric, Rho HeeSool, Song Guang, Paul Corry, Jeong Jun Seop, Lu Kuan-Yi, Chen Chien-Sheng, Zhu Heng
Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
Cold Spring Harb Protoc. 2016 Oct 3;2016(10):2016/10/pdb.top081471. doi: 10.1101/pdb.top081471.
Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the "yeast proteome array," consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein-lipid interactions. Further assays were subsequently developed to allow measurement of protein-DNA, protein-RNA, and protein-protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation. In this introduction, we describe the advent of high-density protein microarrays, as well as current methods for assessing a wide variety of protein interactions and posttranslational modifications.
蛋白质微阵列已成为科学界的一种强大工具，其最大优势在于能够以平行且无偏差的方式进行数千种反应。首个高密度蛋白质微阵列，即所谓的“酵母蛋白质组阵列”，由约5800种全长酵母蛋白质组成，最初用于鉴定蛋白质 - 脂质相互作用。随后又开发了进一步的检测方法，以测量蛋白质 - DNA、蛋白质 - RNA和蛋白质 - 蛋白质相互作用，以及四种著名的翻译后修饰：磷酸化、乙酰化、泛素化和SUMO化。在本引言中，我们描述了高密度蛋白质微阵列的出现，以及目前用于评估多种蛋白质相互作用和翻译后修饰的方法。
