School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Sci Rep. 2016 Oct 4;6:34133. doi: 10.1038/srep34133.
Many members of the genus Artemisia are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used Artemisia species included Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica, and Artemisia atrovirens were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and reliable authentication system to distinguish these commonly used Artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain.
许多艾属植物因其具有多种药理学特性而被广泛用于药用目的。通常，市场上销售的这些草药植物都是经过加工的形式，因此很难进行鉴定。为了确保用于制药产品的原材料适合其预期用途，应对这些草药材料进行常规测试和鉴定。在这项研究中，使用高分辨率熔解（HRM）分析基于内部转录间隔区 2（ITS2）序列分析了五种常用的艾属植物，包括艾蒿、青蒿、薰衣草艾蒿、印度艾蒿和深绿艾蒿。五种密切相关的草药物种的 ITS2 扩增子的熔解曲线明显分离，因此可以通过 HRM 方法进行区分。该方法进一步应用于粉末状商业产品的鉴定。测试的所有商业样本的 HRM 曲线与标签上的植物种类相似。使用邻接法（NJ）树也可以清楚地区分这些同类药用产品。因此，HRM 方法可以提供一种高效可靠的鉴定系统，用于区分市场上常用的艾蒿草药产品，并为药品供应链中的药品质量控制提供技术参考。
