Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea.
PLoS One. 2022 Mar 10;17(3):e0264576. doi: 10.1371/journal.pone.0264576. eCollection 2022.
The genus Artemisia is an important source of medicines in both traditional and modern pharmaceutics, particularly in East Asia. Despite the great benefits of herbal medicine, quality assessment methods for these medicinal herbs are lacking. The young leaves from Artemisia species are generally used, and most of the species have similar morphology, which often leads to adulteration and misuse. This study assembled five complete chloroplast genomes of three Artemisia species, two accessions of A. gmelinii and A. capillaris, and one A. fukudo. Through comparative analysis, we revealed genomic variations and phylogenetic relationships between these species and developed seven InDel-based barcode markers which discriminated the tested species from each other. Additionally, we analyzed specialized metabolites from the species using LC-MS and suggested chemical markers for the identification and authentication of these herbs. We expect that this integrated and complementary authentication method would aid in reducing the misuse of Artemisia species.
蒿属是传统和现代药物学中重要的药用植物来源，特别是在东亚地区。尽管草药有很多好处，但这些草药的质量评估方法却很缺乏。一般使用蒿属植物的嫩叶，而且大多数物种形态相似，这往往导致掺假和误用。本研究组装了三种蒿属植物（两种黄花蒿和青蒿）和一种艾蒿的五个完整的叶绿体基因组。通过比较分析，我们揭示了这些物种之间的基因组变异和系统发育关系，并开发了七种基于插入缺失的条形码标记，可将测试物种相互区分开来。此外，我们还使用 LC-MS 分析了这些物种的特征代谢物，并提出了用于这些草药鉴定和认证的化学标志物。我们期望这种综合互补的鉴定方法有助于减少蒿属植物的误用。
