Rezaei Maryam, Hashemi Mohammad, Sanaei Sara, Mashhadi Mohammad Ali, Hashemi Seyed Mehdi, Bahari Gholamreza, Taheri Mohsen
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Biomed Rep. 2016 Oct;5(4):455-460. doi: 10.3892/br.2016.738. Epub 2016 Aug 8.
Flap endonuclease 1 (FEN1), a DNA repair protein, is important in preventing carcinogenesis. Two functional germ line variants -69G>A (rs174538) and +4150G>T (rs4246215) in the gene have been associated with risk of various types of cancer. The aim of the present study was to evaluate the possible impact of polymorphisms on risk of breast cancer (BC) in a sample of Iranian subjects. The -69G>A and +4150G>T polymorphisms were analyzed in a case-control study that included 266 BC patients and 225 healthy females. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to genotype the variants. The findings demonstrated that the -69G>A and +4150G>T polymorphisms were not associated with BC risk in co-dominant, dominant and recessive inheritance models. The findings indicated that GG/GT, GA/GG and GA/TT genotypes significantly decreased the risk of BC when compared with -69GG/+4150GG. Furthermore, haplotype analysis indicated that -69G/+4150T as well as -69A/+4150G significantly decreased the risk of BC compared with -69G/+4150G. Thus, these findings demonstrated that haplotypes of -69G>A and +4150G>T polymorphisms decreased the risk of BC in an Iranian population. Further studies with larger sample sizes and different ethnicities are required to validate the present findings.
瓣内切核酸酶1（FEN1）是一种DNA修复蛋白，在预防癌症发生中起重要作用。该基因中的两个功能性种系变体-69G>A（rs174538）和+4150G>T（rs4246215）与各种类型癌症的风险相关。本研究的目的是评估多态性对伊朗受试者样本中患乳腺癌（BC）风险的可能影响。在一项病例对照研究中分析了-69G>A和+4150G>T多态性，该研究包括266例BC患者和225名健康女性。采用聚合酶链反应-限制性片段长度多态性分析对变体进行基因分型。研究结果表明，在共显性、显性和隐性遗传模型中，-69G>A和+4150G>T多态性与BC风险无关。研究结果表明，与-69GG/+4150GG相比，GG/GT、GA/GG和GA/TT基因型显著降低了BC风险。此外，单倍型分析表明，与-69G/+4150G相比，-69G/+4150T以及-69A/+4150G显著降低了BC风险。因此，这些研究结果表明，-69G>A和+4150G>T多态性的单倍型降低了伊朗人群患BC的风险。需要进一步开展更大样本量和不同种族的研究来验证本研究结果。
