Lin Kan, Zheng Wei, Lim Chwee Ming, Huang Zhiwei
Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, 117576 Singapore; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, 119260 Singapore.
Department of Otolaryngology, Head and Neck Surgery, National University of Singapore and National University Health System, 119074 Singapore.
Biomed Opt Express. 2016 Aug 26;7(9):3705-3715. doi: 10.1364/BOE.7.003705. eCollection 2016 Sep 1.
We assess the clinical utility of a unique simultaneous fingerprint (FP) (i.e., 800-1800 cm) and high-wavenumber (HW) (i.e., 2800-3600 cm) fiber-optic Raman spectroscopy for diagnosis of laryngeal cancer at endoscopy. A total of 2124 high-quality FP/HW Raman spectra (normal = 1321; cancer = 581) were acquired from 101 tissue sites (normal = 71; cancer = 30) of 60 patients (normal = 44; cancer = 16) undergoing routine endoscopic examination. FP/HW Raman spectra differ significantly between normal and cancerous laryngeal tissue that could be attributed to changes of proteins, lipids, nucleic acids, and the bound water content in the larynx. Partial least squares-discriminant analysis and leave-one tissue site-out, cross-validation were employed on the in vivo FP/HW tissue Raman spectra acquired, yielding a diagnostic accuracy of 91.1% (sensitivity: 93.3% (28/30); specificity: 90.1% (64/71)) for laryngeal cancer identification, which is superior to using either FP (accuracy: 86.1%; sensitivity: 86.7% (26/30); specificity: 85.9% (61/71)) or HW (accuracy: 84.2%; sensitivity: 76.7% (23/30); specificity: 87.3% (62/71)) Raman technique alone. Further receiver operating characteristic analysis reconfirms the best performance of the simultaneous FP/HW Raman technique for laryngeal cancer diagnosis. We demonstrate for the first time that the simultaneous FP/HW Raman spectroscopy technique can be used for improving real-time diagnosis of laryngeal carcinoma during endoscopic examination.
我们评估了一种独特的同时进行指纹区（即800 - 1800厘米）和高波数区（即2800 - 3600厘米）光纤拉曼光谱技术在内镜检查中诊断喉癌的临床实用性。从60例接受常规内镜检查的患者（正常 = 44例；癌症 = 16例）的101个组织部位（正常 = 71个；癌症 = 30个）获取了总共2124条高质量的指纹区/高波数区拉曼光谱。正常和癌性喉组织的指纹区/高波数区拉曼光谱存在显著差异，这可归因于喉部蛋白质、脂质、核酸和结合水含量的变化。对获取的体内指纹区/高波数区组织拉曼光谱采用偏最小二乘判别分析和留一组织部位法交叉验证，在识别喉癌方面诊断准确率为91.1%（敏感性：93.3%（28/30）；特异性：90.1%（64/71）），优于单独使用指纹区（准确率：86.1%；敏感性：86.7%（26/30）；特异性：85.9%（61/71））或高波数区（准确率：84.2%；敏感性：76.7%（23/30）；特异性：87.3%（62/71））拉曼技术。进一步的受试者工作特征分析再次证实了同时进行指纹区/高波数区拉曼技术在喉癌诊断中的最佳性能。我们首次证明，同时进行指纹区/高波数区拉曼光谱技术可用于在内镜检查期间改善喉癌的实时诊断。
