Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore.
Department of Otolaryngology, Singapore General Hospital, Singapore 169608, Singapore.
Biosens Bioelectron. 2012 May 15;35(1):213-217. doi: 10.1016/j.bios.2012.02.050. Epub 2012 Mar 15.
We report the implementation of the transnasal image-guided high wavenumber (HW) Raman spectroscopy to differentiate tumor from normal laryngeal tissue at endoscopy. A rapid-acquisition Raman spectroscopy system coupled with a miniaturized fiber-optic Raman probe was utilized to realize real-time HW Raman (2800-3020 cm(-1)) measurements in the larynx. A total of 94 HW Raman spectra (22 normal sites, 72 tumor sites) were acquired from 39 patients who underwent laryngoscopic screening. Significant differences in Raman intensities of prominent Raman bands at 2845, 2880 and 2920 cm(-1) (CH(2) stretching of lipids), and 2940 cm(-1) (CH(3) stretching of proteins) were observed between normal and cancer laryngeal tissue. The diagnostic algorithms based on principal components analysis (PCA) and linear discriminant analysis (LDA) together with the leave-one subject-out, cross-validation method on HW Raman spectra yielded a diagnostic sensitivity of 90.3% (65/72) and specificity of 90.9% (20/22) for laryngeal cancer identification. This study demonstrates that HW Raman spectroscopy has the potential for the noninvasive, real-time diagnosis and detection of laryngeal cancer at the molecular level.
我们报告了经鼻内镜引导的高波数（HW）拉曼光谱技术在区分肿瘤与正常喉组织中的应用。我们使用快速采集拉曼光谱系统和微型光纤拉曼探头，实现了喉部实时 HW 拉曼（2800-3020cm(-1)) 测量。共对 39 名接受喉镜筛查的患者的 94 个 HW 拉曼光谱（22 个正常部位，72 个肿瘤部位）进行了采集。在正常和癌症喉组织中，在 2845、2880 和 2920cm(-1)（脂质 CH(2)伸缩）和 2940cm(-1)（蛋白质 CH(3)伸缩）处的显著拉曼带强度差异明显。基于主成分分析（PCA）和线性判别分析（LDA）的诊断算法，以及对 HW 拉曼光谱的逐个受试者外留一交叉验证方法，得出了用于喉癌识别的诊断灵敏度为 90.3%（65/72）和特异性为 90.9%（20/22）。本研究表明，HW 拉曼光谱具有在分子水平上进行非侵入性、实时诊断和检测喉癌的潜力。
