Katayama S, Shionoya H, Ohtake S
Microbiol Immunol. 1978;22(2):89-101. doi: 10.1111/j.1348-0421.1978.tb00352.x.
A method for quantitative extraction of extravasated dye from the skin was studied in guinea pigs and rats. A simple method with a low cost and good recovery was established as follows; A piece of the skin containing extravasated dye was soaked overnight in a stoppered glass tube containing 1 ml of 1 N KOH at 37 C. Then, 9 ml of mixed solution of 0.6 N H3PO4 and acetone (5:13) was added to the tube. The tube was shaken vigorously for a few seconds and centrifuged at 3,000 rpm for 15 min. Absorbance of supernatant was measured at 620 nm. The recovery rate of the dye was about 95 per cent both in guinea pigs and rats. Using this method we observed that fasting stress significantly reduced the intensity of skin reaction induced by chemical mediators, heterologous PCA and especially homologous PCA in guinea pigs.
在豚鼠和大鼠身上研究了一种从皮肤中定量提取外渗染料的方法。建立了一种低成本且回收率高的简单方法如下：将含有外渗染料的一块皮肤在37℃下于装有1ml 1N KOH的带塞玻璃管中浸泡过夜。然后，向管中加入9ml 0.6N H3PO4和丙酮（5:13）的混合溶液。将管剧烈振荡几秒钟，然后以3000转/分钟的速度离心15分钟。在620nm处测量上清液的吸光度。豚鼠和大鼠中染料的回收率均约为95%。使用该方法我们观察到禁食应激显著降低了化学介质、异种被动皮肤过敏反应（PCA）尤其是豚鼠同种PCA诱导的皮肤反应强度。
