Girmay Zenebe, Gorems Weldesemayat, Birhanu Getachew, Zewdie Solomon
School of Forestry, Wondo Genet College of Forestry and Natural Resource, Hawassa University, P.O. Box 128, Shashemene, Ethiopia.
Life and Earth Sciences Institute, Pan African University, University of Ibadan, Ibadan, Nigeria.
AMB Express. 2016 Dec;6(1):87. doi: 10.1186/s13568-016-0265-1. Epub 2016 Oct 4.
Mushroom cultivation is reported as an economically viable bio-technology process for conversion of various lignocellulosic wastes. Given the lack of technology know-how on the cultivation of mushroom, this study was conducted in Wondo Genet College of Forestry and Natural Resource, with the aim to assess the suitability of selected substrates (agricultural and/or forest wastes) for oyster mushroom cultivation. Accordingly, four substrates (cotton seed, paper waste, wheat straw, and sawdust) were tested for their efficacy in oyster mushroom production. Pure culture of oyster mushroom was obtained from Mycology laboratory, Department of Plant Biology and Biodiversity Management, Addis Ababa University. The pure culture was inoculated on potato dextrose agar for spawn preparation. Then, the spawn containing sorghum was inoculated with the fungal culture for the formation of fruiting bodies on the agricultural wastes. The oyster mushroom cultivation was undertaken under aseptic conditions, and the growth and development of mushroom were monitored daily. Results of the study revealed that oyster mushroom can grow on cotton seed, paper waste, sawdust and wheat straw, with varying growth performances. The highest biological and economic yield, as well as the highest percentage of biological efficiency of oyster mushroom was obtained from cotton seed, while the least was from sawdust. The study recommends cotton seed, followed by paper waste as suitable substrates for the cultivation of oyster mushroom. It also suggests that there is a need for further investigation on various aspects of oyster mushroom cultivation in Ethiopia to promote the industry.
蘑菇栽培被认为是一种将各种木质纤维素废物转化为经济可行的生物技术过程。鉴于缺乏蘑菇栽培技术知识，本研究在翁多杰内特林业与自然资源学院开展，旨在评估选定的基质（农业和/或森林废物）对平菇栽培的适用性。据此，对四种基质（棉籽、废纸、麦秸和锯末）在平菇生产中的功效进行了测试。平菇的纯培养物取自亚的斯亚贝巴大学植物生物学与生物多样性管理系的真菌学实验室。将纯培养物接种在马铃薯葡萄糖琼脂上制备菌种。然后，将含有高粱的菌种接种真菌培养物，以便在农业废物上形成子实体。平菇栽培在无菌条件下进行，每天监测蘑菇的生长和发育情况。研究结果表明，平菇能够在棉籽、废纸、锯末和麦秸上生长，生长表现各异。平菇的最高生物学产量、经济产量以及最高生物效率百分比均来自棉籽，而最低的来自锯末。该研究推荐棉籽，其次是废纸，作为平菇栽培的适宜基质。研究还表明，有必要对埃塞俄比亚平菇栽培的各个方面进行进一步调查，以促进该产业发展。
