黑曲霉在转鼓反应器中同时产糖化酶和外切多聚半乳糖醛酸酶
Simultaneous Production of Amyloglucosidase and Exo-Polygalacturonase by Aspergillus niger in a Rotating Drum Reactor.
作者信息
Colla Eliane, Santos Lucielen Oliveira, Deamici Kricelle, Magagnin Glênio, Vendruscolo Mauricio, Costa Jorge Alberto Vieira
机构信息
Graduate Program in Food Technology, Federal Technological University of Paraná (UTFPR), 4230 Brasil Avenue, P.O. Box: 271, Medianeira, PR, Postal Code 85.884-000, Brazil.
Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil.
出版信息
Appl Biochem Biotechnol. 2017 Feb;181(2):627-637. doi: 10.1007/s12010-016-2237-y. Epub 2016 Oct 4.
Simultaneous production of amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) was carried out by Aspergillus niger in substrate of defatted rice bran in a rotating drum bioreactor (RDB) and studied by a 3 × 2 factorial experimental design. Variables under study were A. niger strains (A. niger NRRL 3122 and A. niger t0005/007-2), types of inoculum (spore suspension and fermented bran), and types of inducer (starch, pectin, and a mix of both). Solid-state fermentation process (SSF) was conducted at 30 °C under 60-vvm aeration for 96 h in a pilot scale. Production of AMG and exo-PG was significantly affected by the fungal strain and the type of inoculum, but inducers did not trigger any significant effect, an evidence of the fact that these enzymes are constitutive. The maximum activity of exo-PG was 84 U g whereas the maximum yield of AMG was 886.25 U g.
黑曲霉在转鼓式生物反应器（RDB）中以脱脂米糠为底物同时生产淀粉葡萄糖苷酶（AMG）和外切多聚半乳糖醛酸酶（exo-PG），并通过3×2析因实验设计进行研究。所研究的变量包括黑曲霉菌株（黑曲霉NRRL 3122和黑曲霉t0005/007-2）、接种物类型（孢子悬浮液和发酵麸皮）以及诱导剂类型（淀粉、果胶和两者的混合物）。固态发酵过程（SSF）在中试规模下于30℃、60 vvm通气条件下进行96小时。AMG和exo-PG的产生受到真菌菌株和接种物类型的显著影响，但诱导剂未引发任何显著影响，这证明这些酶是组成型的。exo-PG的最大活性为84 U/g，而AMG的最大产量为886.25 U/g。
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