Patil Sarvamangala R, Dayanand A
Department of Microbiology, Vishweshwariah College of Applied Sciences, Gulbarga 585102, India.
Bioresour Technol. 2006 Nov;97(16):2054-8. doi: 10.1016/j.biortech.2005.09.015. Epub 2005 Nov 2.
Studies were carried out on the production of pectinases using deseeded sunflower head by Aspergillus niger DMF 27 and DMF 45 in submerged fermentation (SmF) and solid-state fermentation (SSF). Higher titres of endo- and exo-pectinases were observed when medium was supplemented with carbon (4% glucose for SmF and 6% sucrose for SSF) and nitrogen (ammonium sulphate, 0.3% for both SmF and SSF) sources. Green gram husk proved to be relatively a better supplement to attain higher yield of endo-pectinase (11.7 U/g) and exo-pectinase (30.0 U/g) in solid-state conditions. Maximum production of endo-pectinase (19.8 U/g) and exo-pectinase (45.9 U/g) by DMF 45 were recorded in SSF when compared to endo-pectinase (18.9 U/ml) and exo-pectinase (30.3 U/ml) by DMF 27 in SmF under optimum process conditions.
利用黑曲霉DMF 27和DMF 45，在液体深层发酵（SmF）和固态发酵（SSF）条件下，以去籽向日葵花盘为原料生产果胶酶进行了相关研究。当培养基添加碳源（SmF为4%葡萄糖，SSF为6%蔗糖）和氮源（硫酸铵，SmF和SSF均为0.3%）时，内切和外切果胶酶的产量更高。在固态条件下，绿豆皮被证明是相对较好的补充物，可获得更高产量的内切果胶酶（11.7 U/g）和外切果胶酶（30.0 U/g）。在最佳工艺条件下，与DMF 27在SmF中产生的内切果胶酶（18.9 U/ml）和外切果胶酶（30.3 U/ml）相比，DMF 45在SSF中产生的内切果胶酶（19.8 U/g）和外切果胶酶（45.9 U/g）产量最高。
