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[鼬科动物中重复序列的EcoRI和BamHI家族]

[EcoRI- and BamHI-families of repeated sequences in mustelids].

作者信息

Lushnikova T P, Grafodatskiĭ A S, Ivanov S V, Romashchenko A G, Ternovskiĭ L V, Ternovskaia Iu G, Radzhabli S I

出版信息

Genetika. 1989 Aug;25(8):1449-61.

PMID:2583487
Abstract

The restriction enzymes EcoRI and BamHI digest the genomic DNAs from six mustelids species Mustela lutreola, M. vision, M. erminea, M. sibirica, Vormela peregusna, producing repeated fragments varying in length. Some fragments were hybridized to chromosomes and restriction digests of DNAs from some mustelids and other mammals. The 0.7 kb EcoRI repeats from DNA of M. erminea are dispersed over chromosomes of carnivors. The 1.35, 1.9 and 2.7 kb BamHI repeats from DNA of polecat M. putorius furo are specific for mustelids. These repeats demonstrate interspecific variation in length and the number of copies. All BamHI repeats have no strict tandem organization. The 1.9 kb BamHI repeats are concentrated in the heterochromatic pericentromeric regions and additional chromosome arms. The 1.35 kb BamHI repeats are only located in the centromeric regions of chromosomes of five species and are absent in Vormela peregusna.

摘要

限制性内切酶EcoRI和BamHI消化六种鼬科动物(欧洲水貂、伶鼬、白鼬、黄鼬、艾虎)的基因组DNA,产生长度各异的重复片段。一些片段与某些鼬科动物和其他哺乳动物的染色体及DNA限制性消化产物杂交。白鼬DNA中0.7 kb的EcoRI重复序列分散在食肉动物的染色体上。鸡貂DNA中1.35 kb、1.9 kb和2.7 kb的BamHI重复序列是鼬科动物特有的。这些重复序列在长度和拷贝数上表现出种间差异。所有BamHI重复序列都没有严格的串联组织。1.9 kb的BamHI重复序列集中在异染色质的着丝粒周围区域和其他染色体臂上。1.35 kb的BamHI重复序列仅位于五个物种染色体的着丝粒区域,在艾虎中不存在。

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