Maughan R J, Donnelly A E, Gleeson M, Whiting P H, Walker K A, Clough P J
Department of Environmental & Occupational Medicine, University Medical School, Aberdeen, UK.
Muscle Nerve. 1989 Apr;12(4):332-6. doi: 10.1002/mus.880120412.
Lipid peroxidation initiated by free radical reactions is associated with tissue necrosis in a variety of conditions. We have measured serum lipid peroxide concentrations (as total thiobarbituric acid-reactive substances, TBARS) and creatine kinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) activities and subjective muscle soreness in 16 men before and after a 45-minute downhill treadmill run. TBARS concentrations and enzyme activities were increased after exercise, with peak values observed at 6 hours (TBARS, LDH) or 24 hours (CK, AST) after exercise. Serum LDH activity returned to preexercise levels by 48 hours after exercise and TBARS by 72 hours after exercise: CK and AST activities remained elevated 72 hours after exercise. Leg muscle soreness also increased, with the greatest degree of soreness seen at 24 or 48 hours after exercise. Subjects with the greatest increase in CK, LDH, and AST also showed the highest serum TBARS concentration. This suggests a possible relationship between free radical generation and exercise-induced muscle damage.
由自由基反应引发的脂质过氧化与多种情况下的组织坏死有关。我们测量了16名男性在进行45分钟下坡跑步机跑步前后的血清脂质过氧化物浓度（以总硫代巴比妥酸反应性物质，即TBARS表示）、肌酸激酶（CK）、乳酸脱氢酶（LDH）和天冬氨酸转氨酶（AST）活性以及主观肌肉酸痛情况。运动后TBARS浓度和酶活性升高，在运动后6小时（TBARS、LDH）或24小时（CK、AST）观察到峰值。运动后48小时血清LDH活性恢复到运动前水平，运动后72小时TBARS恢复到运动前水平：运动后72小时CK和AST活性仍升高。腿部肌肉酸痛也增加，在运动后24或48小时酸痛程度最大。CK、LDH和AST升高幅度最大的受试者血清TBARS浓度也最高。这表明自由基生成与运动诱导的肌肉损伤之间可能存在关联。
