Zhang L, Kong X-J, Wang Z-Q, Xu F-S, Zhu Y-T
Yi- Tang Zhu, Department of Clinical Laboratory; Cangzhou Central Hospital of Hebei Province, No.16 Xinhuaxi Road, Cangzhou City 061001, Hebei Province, P.R.China, Tel and Fax: +86-0137-2075536, Email:
J Nutr Health Aging. 2016;20(8):835-840. doi: 10.1007/s12603-016-0723-0.
The present study was aimed to study the neuroprotective therapeutic effect of curcumin on the male albino rat brain. Subarachnoid hemorrhage leads to severe mortality rate and morbidity, and oxidative stress is a crucial factor in subarachnoid hemorrhage. Therefore, we investigated the effect of curcumin on oxidative stress and glutamate and glutamate transporter-1 on a subarachnoid hemorrhage-induced male albino rats. The curcumin commonly used for the treatment and saline used for the control. Curcumin (10 mg/kg bwt) dissolved in saline and administered orally to the rats for one week. Glutamate, glutamate transporter-1, malondialdehyde (MDA), superoxide dismutase (SOD), catalase, glutathione reductase and lactate dehydrogenase (LDH) activities were determined. Glutamate level was lower in the curcumin-treated rats compared to their respective controls. Glutamate transporter-1 did not alter in the curcumin-treated rats compared to their controls. Glutamate transporter-1 protein expression is significantly reduced in the curcumin-treated rats. MDA levels decreased 18 and 29 % in the hippocampus and the cortex region respectively. SOD (17% and 32%), and catalase (19% and 24%) activities were increased in the curcumin-treated hippocampus and the cortex region respectively. Glutathione reductase (13% and 19%) and LDH (21% and 30%) activities were increased in the treated hippocampus and the cortex region respectively. The mRNA expression of NK-kB and TLR4 was significantly reduced following curcumin treatment. Taking all these data together, the curcumin found to be effective against oxidative stress and glutamate neurotoxicity in the male albino rats.
本研究旨在探讨姜黄素对雄性白化大鼠脑的神经保护治疗作用。蛛网膜下腔出血导致严重的死亡率和发病率，氧化应激是蛛网膜下腔出血的关键因素。因此，我们研究了姜黄素对蛛网膜下腔出血诱导的雄性白化大鼠氧化应激、谷氨酸和谷氨酸转运体-1的影响。将常用的姜黄素用于治疗，生理盐水用于对照。将姜黄素（10mg/kg体重）溶解于生理盐水中，口服给药大鼠一周。测定谷氨酸、谷氨酸转运体-1、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽还原酶和乳酸脱氢酶（LDH）的活性。与各自的对照组相比，姜黄素治疗组大鼠的谷氨酸水平较低。与对照组相比，姜黄素治疗组大鼠的谷氨酸转运体-1没有改变。姜黄素治疗组大鼠的谷氨酸转运体-1蛋白表达显著降低。海马和皮质区域的MDA水平分别下降了18%和29%。姜黄素治疗组海马和皮质区域的SOD活性（分别为17%和32%）和过氧化氢酶活性（分别为19%和24%）分别增加。治疗组海马和皮质区域的谷胱甘肽还原酶活性（分别为13%和19%）和LDH活性（分别为21%和30%）分别增加。姜黄素治疗后，NK-kB和TLR4的mRNA表达显著降低。综合所有这些数据，发现姜黄素对雄性白化大鼠的氧化应激和谷氨酸神经毒性有效。
