Germroth P, Schwerdtfeger W K, Buhl E H
Max-Planck-Institut für Hirnforschung, Frankfurt/M. F.R.G.
Neuroscience. 1989;30(3):683-91. doi: 10.1016/0306-4522(89)90161-9.
Cells of origin of the entorhinohippocampal pathway were retrogradely labeled by injection of Fast Blue into the ipsilateral hippocampus. The cells, which were located in layers I, II and III of the lateral entorhinal cortex, were then intracellularly injected with Lucifer Yellow to reveal their complete morphology. We could thus establish that among the hippocampally projecting entorhinal cells there are pyramidal and pyramid-like cells, spiny stellate cells of various shapes, sparsely spinous horizontal and multipolar cells. The involvement of horizontal and multipolar neurons in projections has not previously been recognized although all of these cell types have already been described in Golgi studies. The results indicate that the organization of the perforant path is more complex than has been assumed. Finally, they are at variance with the classical concept which subdivides cortical neurons into projection neurons (pyramidal and spiny stellate) and interneurons (non-pyramidal, local circuit neurons).
通过向同侧海马注射快蓝，逆行标记内嗅海马通路的起源细胞。然后，对位于外侧内嗅皮质第I、II和III层的这些细胞进行细胞内注射路西法黄，以揭示其完整形态。由此我们可以确定，在投射到海马的内嗅细胞中，有锥体细胞和类锥体细胞、各种形状的棘状星形细胞、棘突稀疏的水平细胞和多极细胞。尽管所有这些细胞类型在高尔基研究中都已被描述，但水平神经元和多极神经元参与投射这一点此前尚未被认识到。结果表明，穿通通路的组织比之前所认为的更为复杂。最后，它们与将皮质神经元细分为投射神经元（锥体细胞和棘状星形细胞）和中间神经元（非锥体细胞，局部回路神经元）的经典概念不一致。
