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从念珠菌血症中分离出的菌种的序列鉴定

Sequence-identification of species isolated from candidemia.

作者信息

Fathi Naeimeh, Mohammadi Rasoul, Tabatabaiefar Mohammad Amin, Ghahri Mohammad, Sadrossadati Seyedeh Zahra

机构信息

Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Adv Biomed Res. 2016 Sep 26;5:150. doi: 10.4103/2277-9175.188485. eCollection 2016.

Abstract

BACKGROUND

species are the most prevalent cause of invasive fungal infections such as candidemia. Candidemia is a lethal fungal infection among immunocompromised patients worldwide. Main pathogen is but a global shift in epidemiology toward non- species have reported. Species identification is imperative for good management of candidemia as a fatal infection. The aim of the study is to identify spp. obtained from candidemia and determination of mortality rate among this population.

MATERIALS AND METHODS

The study was performed during February 2014 to March 2015 in Tehran, Iran. Two-hundred and four blood cultures were evaluated for fungal bloodstream infection. Identification of isolates was carried out using phenotypic tests and polymerase chain reaction sequencing technique.

RESULTS

Twenty-two out of 204 patients (10.8%) had candidemia. was the most prevalent species (45.4%), followed by (31.8%) and (22.7%). Male to female sex ratio was 8/14.

CONCLUSIONS

The emergence of resistant strains of species should be considered by physicians to decrease the mortality of this fatal fungal infection by appropriate treatment.

摘要

背景

念珠菌属是侵袭性真菌感染(如念珠菌血症)最常见的病因。念珠菌血症是全球免疫功能低下患者中一种致命的真菌感染。主要病原体是 ,但已有报道称全球念珠菌血症的流行病学正朝着非 属菌种转变。对于作为致命感染的念珠菌血症的良好管理而言,菌种鉴定至关重要。本研究的目的是鉴定从念珠菌血症患者中分离出的念珠菌属菌种,并确定该人群的死亡率。

材料与方法

本研究于2014年2月至2015年3月在伊朗德黑兰进行。对204份血培养物进行了真菌血流感染评估。使用表型试验和聚合酶链反应测序技术对分离株进行鉴定。

结果

204例患者中有22例(10.8%)发生念珠菌血症。 是最常见的菌种(45.4%),其次是 (31.8%)和 (22.7%)。男女比例为8/14。

结论

医生应考虑 属菌种耐药菌株的出现,通过适当治疗降低这种致命真菌感染的死亡率。

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Sequence-identification of species isolated from candidemia.从念珠菌血症中分离出的菌种的序列鉴定
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National surveillance of fungemia in Denmark (2004 to 2009).丹麦的真菌血症全国监测(2004 年至 2009 年)。
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