Wernicke Anna-Katharina, Churin Yuri, Sheridan Diana, Windhorst Anita, Tschuschner Annette, Gattenlöhner Stefan, Roderfeld Martin, Roeb Elke
Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
Department of Pathology, Justus-Liebig-University, Giessen, Germany.
Oncotarget. 2016 Nov 8;7(45):73552-73557. doi: 10.18632/oncotarget.12429.
An exact classification of precancerous stages of colorectal polyps might improve therapy and patients´ outcome. Here we investigate the association between grade of dysplasia and Matrix metalloproteinase-13 (MMP-13) expression in 137 biopsies from patients with cancerous and non-cancerous colorectal adenomas. A reproducible staining procedure for histologic MMP-13 analysis in routinely fixed colorectal biopsy specimens has been established. A newly adopted immunoreactive scoring system for MMP-13 was demonstrated as reliable readout.The strength of the association between pathologic stage and immunoreactive MMP-13 scoring emphasizes its eligibility for diagnosis in precancerous colorectal lesions.
对结直肠息肉癌前阶段进行精确分类可能会改善治疗效果和患者预后。在此，我们研究了137例患有癌性和非癌性结肠直肠腺瘤患者活检组织中发育异常分级与基质金属蛋白酶-13（MMP-13）表达之间的关联。已建立了一种可重复的染色程序，用于常规固定的结肠直肠活检标本中组织学MMP-13分析。一种新采用的MMP-13免疫反应评分系统被证明是可靠的读数。病理分期与免疫反应性MMP-13评分之间关联的强度强调了其在结直肠癌前病变诊断中的适用性。
