Department of Gastrointestinal Surgery, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama-shi, Kanagawa-ken 241-0815, Japan.
Anticancer Res. 2010 Jul;30(7):2693-9.
Matrix metalloproteinase-7 (MMP-7), MMP-9, MMP-13, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are considered to have important roles in the invasiveness and outcomes of colorectal cancer (CRC). This study examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially as related to liver metastasis. The study analysed surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated colorectal cancer. MMP-7, MMP-9, MMP-13, TIMP-1, and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time, reverse-transcriptase polymerase chain reaction. Expression levels of MMP-7, MMP-9, MMP-13 and TIMP-1 were higher in cancer tissue than in adjacent normal mucosa. On analysis of the relations between gene expression and clinicopathological factors, MMP-13 expression was found to correlate with liver metastasis. Moreover, MMP-13 expression levels were higher in tumour tissue with liver metastasis than in that without liver metastasis. It is concluded that MMP-13 gene expression is a useful predictor of liver metastasis in patients with CRC.
基质金属蛋白酶-7（MMP-7）、MMP-9、MMP-13 和基质金属蛋白酶组织抑制剂-1（TIMP-1）被认为在结直肠癌（CRC）的侵袭性和结果中具有重要作用。本研究探讨了这些基因在结直肠癌患者中的相对表达的临床病理意义，特别是与肝转移的关系。该研究分析了 202 例未经治疗的结直肠癌患者手术切除的癌组织和相邻正常粘膜标本。通过定量实时逆转录聚合酶链反应检测癌组织和相邻正常粘膜中的 MMP-7、MMP-9、MMP-13、TIMP-1 和β-肌动蛋白 mRNA。癌组织中 MMP-7、MMP-9、MMP-13 和 TIMP-1 的表达水平高于相邻正常粘膜。在分析基因表达与临床病理因素之间的关系时，发现 MMP-13 的表达与肝转移有关。此外，有肝转移的肿瘤组织中 MMP-13 的表达水平高于无肝转移的肿瘤组织。结论：MMP-13 基因表达是预测 CRC 患者肝转移的有用指标。
