Jian Huahua, Li Shengkang, Tang Xixiang, Xiao Xiang
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, PR China; Marine Biology Institute, Shantou University, Shantou, PR China.
Mar Genomics. 2016 Dec;30:87-91. doi: 10.1016/j.margen.2016.09.004. Epub 2016 Oct 4.
Low temperature and high hydrostatic pressure (HHP) are two of the most remarkable environmental factors influencing deep-sea ecosystem. The adaptive mechanisms of microorganisms which live in these extreme environments to low temperature and high pressure warrant investigation. In this study, the global gene expression patterns of the deep-sea bacterium Shewanella piezotolerans WP3 in response to cold (0 °C) and HHP (50 MPa) shock were evaluated through DNA microarray analysis. Results revealed that 22, 66, and 106 genes were differentially expressed after WP3 was respectively exposed to cold shock for 30, 60, and 90 min. Of these genes, 16 genes were identified as common differentially expressed genes (DEGs). After 30 min and 120 min of HHP shock, 5 and 10 genes were respectively identified as DEGs. The hierarchical clustering analysis of the DEG pattern indicated that WP3 may employ different adaptive strategies to cope with cold and HHP shock stress. Taken together, our study provided a transcriptome resource for deep-sea bacterial responses to cold and HHP stress. This study also established a basis for further investigations on environmental adaptive mechanisms utilized by benthic bacteria.
低温和高静水压力（HHP）是影响深海生态系统的两个最显著的环境因素。生活在这些极端环境中的微生物对低温和高压的适应机制值得研究。在本研究中，通过DNA微阵列分析评估了深海细菌耐压希瓦氏菌WP3对冷休克（0°C）和HHP休克（50 MPa）的全局基因表达模式。结果显示，WP3分别暴露于冷休克30、60和90分钟后，有22、66和106个基因差异表达。在这些基因中，有16个基因被鉴定为共同差异表达基因（DEG）。在HHP休克30分钟和120分钟后，分别有5个和10个基因被鉴定为DEG。DEG模式的层次聚类分析表明，WP3可能采用不同的适应策略来应对冷休克和HHP休克压力。综上所述，我们的研究为深海细菌对冷和HHP压力的反应提供了一个转录组资源。本研究也为进一步研究底栖细菌利用的环境适应机制奠定了基础。
