Nawaz Muhammad Z, Jian Huahua, He Ying, Xiong Lei, Xiao Xiang, Wang Fengping
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityShanghai, China.
State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong UniversityShanghai, China.
Front Microbiol. 2017 Jun 15;8:1093. doi: 10.3389/fmicb.2017.01093. eCollection 2017.
are one of the most abundant Proteobacteria in the deep-sea and are renowned for their versatile electron accepting capacities. The molecular mechanisms involved in their adaptation to diverse and extreme environments are not well understood. Small non-coding RNAs (sRNAs) are known for modulating the gene expression at transcriptional and posttranscriptional levels, subsequently playing a key role in microbial adaptation. To understand the potential roles of sRNAs in the adaptation of toward deep-sea environments, here an approach was utilized to detect the sRNAs in the genome of WP3, a piezotolerant and psychrotolerant deep-sea iron reducing bacterium. After scanning 3673 sets of 5' and 3' UTRs of orthologous genes, 209 sRNA candidates were identified with high confidence in WP3. About 92% (193 out of 209) of these putative sRNAs belong to the class -encoded RNAs, suggesting that -regulatory RNAs are the dominant class of sRNAs in WP3. The remaining 16 -regulatory RNAs were validated through quantitative polymerase chain reaction. Five -sRNAs were further shown to act as cold regulated sRNAs. Our study provided additional evidence at the transcriptional level to decipher the microbial adaptation mechanisms to extreme environmental conditions.
是深海中最丰富的变形菌之一，以其多样的电子接受能力而闻名。它们适应多样和极端环境所涉及的分子机制尚未得到充分了解。小非编码RNA（sRNA）以在转录和转录后水平调节基因表达而闻名，随后在微生物适应中发挥关键作用。为了了解sRNA在适应深海环境中的潜在作用，本文采用一种方法来检测耐压耐冷深海铁还原细菌WP3基因组中的sRNA。在扫描了3673组直系同源基因的5'和3'UTR后，在WP3中高可信度地鉴定出209个sRNA候选物。这些假定的sRNA中约92%（209个中的193个）属于类编码RNA，这表明调控RNA是WP3中sRNA的主要类别。其余16个调控RNA通过定量聚合酶链反应得到验证。进一步证明有5个sRNA作为冷调节sRNA起作用。我们的研究在转录水平上提供了额外的证据，以破译微生物对极端环境条件的适应机制。
