Akhter S, Rakha B A, Ansari M S, Iqbal S, Khalid M
Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan.
Department of Wildlife Management Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan.
Theriogenology. 2018 Oct 1;119:137-142. doi: 10.1016/j.theriogenology.2018.06.019. Epub 2018 Jun 23.
Egg yolk is used as a cryoprotectant in semen preservation. However, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. Therefore, study was conducted to identify the optimum level of pigeon egg yolk (PEY) in Tris citric acid (TCA) extender for freezability and in vivo fertility of buffalo semen. Semen was collected at weekly intervals for a period of three weeks (replicates) from 6 Nili Ravi buffalo bulls (2 ejaculates/bull/replicate) and diluted with TCA extender (50 × 10 motile spermatozoa ml) containing 5%, 10%, 15% and 20% PEY or 20% CEY (control) and cryopreserved. Post-thaw sperm quality and extracellular enzymes leakage was assessed after thawing. Sperm motility, plasma membrane integrity, livability and viability was significantly higher in extenders containing 10% and 15% PEY compared to 5% PEY, 20% PEY or 20% CEY (controls). A dose-dependent decrease was recorded in the chromatin damage for the PEY, being lowest for the 15% and 20% PEY which was significantly less compared to controls (20% CEY). The extracellular GOT and LDH leakage was significantly lower (P < 0.05) in extender containing 10% and 15% PEY compared to the controls. Semen collected from 2 bulls, cryopreserved in extenders containing 15% PEY or 20% chicken egg yolk was assessed for fertility after artificial inseminations. A total of 400 buffaloes were inseminated (100 inseminations/extender/bull). The overall fertility rate was significantly higher (P < 0.05) with semen cryopreserved in extender containing 15% PEY (56%) compared to 20% CEY (42%; controls). In conclusion, pigeon egg yolk at 15% offers advantages over 20% chicken egg yolk in terms of in vitro post-thaw semen quality and in vivo fertility of buffalo.
蛋黄被用作精液保存中的冷冻保护剂。然而，其成分因物种而异，这可能会影响其在冻融过程中的有效性。因此，本研究旨在确定在柠檬酸三羟甲基氨基甲烷（TCA）稀释液中，鸽蛋黄（PEY）的最佳添加水平，以提高水牛精液的冷冻性能和体内受精能力。每隔一周从6头尼里-拉菲水牛公牛身上采集精液，为期三周（重复采样）（每头公牛每次重复采集2份射精精液），并用含有5%、10%、15%和20% PEY或20%鸡蛋黄（CEY，作为对照）的TCA稀释液（50×10⁶ 个活动精子/毫升）进行稀释，然后进行冷冻保存。解冻后评估解冻后精子质量和细胞外酶泄漏情况。与含有5% PEY、20% PEY或20% CEY（对照）的稀释液相比，含有10%和15% PEY的稀释液中的精子活力、质膜完整性、存活率和生存能力显著更高。记录到PEY组染色质损伤呈剂量依赖性下降，15%和20% PEY组最低，与对照组（20% CEY）相比显著更低。与对照组相比，含有10%和15% PEY的稀释液中细胞外谷草转氨酶（GOT）和乳酸脱氢酶（LDH）泄漏显著更低（P<0.05）。从2头公牛采集的精液，用含有15% PEY或20%鸡蛋黄的稀释液冷冻保存后，进行人工授精评估其生育能力。总共对400头水牛进行了授精（每种稀释液/每头公牛进行100次授精）。与20% CEY（42%，对照）相比，用含有15% PEY的稀释液冷冻保存的精液总体受胎率显著更高（P<0.05）（56%）。总之，就体外解冻后精液质量和水牛体内受精能力而言，15%的鸽蛋黄比20%的鸡蛋黄具有优势。
