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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Lembeck F, Mayer N, Schindler G

Naunyn Schmiedebergs Arch Pharmacol. 1978 May;303(1):79-86. doi: 10.1007/BF00496188.

Substance P (SP) could be extracted from brain homogenates with chloroform-methanol by a method which extracts all lipids. 2. SP could be transferred form this total lipid extract (TLE) into an aqueous solution at low pH values (2.0--3.0). 3. At higher pH values (5.5) SP could be transferred from an aqueous phase into an organic phase (chloroform:methanol, 2:1) and recombined with TLE (which was previously freed from endogenous SP) contained in this phase. The binding capacity of TLE for SP exceeded by far the amount of endogenous SP bound originally in the brain extracts. 4. Among the lipids present in TLE, phosphatidylserine was able to bind and release SP in a pH dependent manner. 5. It is suggested that SP bound to phosphatidylserine is the storage form of SP in the brain. The mechanisms by which it is released are still unknown. The possibility that the SP-receptor is also a phospholipid is considered.

可以通过一种能提取所有脂质的方法，用氯仿 - 甲醇从脑匀浆中提取P物质（SP）。2. 在低pH值（2.0 - 3.0）下，SP可以从这种总脂质提取物（TLE）转移到水溶液中。3. 在较高pH值（5.5）时，SP可以从水相转移到有机相（氯仿：甲醇，2:1）中，并与该相中所含的TLE（先前已去除内源性SP）重新结合。TLE对SP的结合能力远远超过脑提取物中最初结合的内源性SP的量。4. 在TLE中存在的脂质中，磷脂酰丝氨酸能够以pH依赖的方式结合和释放SP。5. 有人提出，与磷脂酰丝氨酸结合的SP是脑中SP的储存形式。其释放的机制仍然未知。还考虑了SP受体也是一种磷脂的可能性。

Lembeck F, Mayer N, Schindler G

Naunyn Schmiedebergs Arch Pharmacol. 1978 May;303(1):79-86. doi: 10.1007/BF00496188.

Substance P (SP) could be extracted from brain homogenates with chloroform-methanol by a method which extracts all lipids. 2. SP could be transferred form this total lipid extract (TLE) into an aqueous solution at low pH values (2.0--3.0). 3. At higher pH values (5.5) SP could be transferred from an aqueous phase into an organic phase (chloroform:methanol, 2:1) and recombined with TLE (which was previously freed from endogenous SP) contained in this phase. The binding capacity of TLE for SP exceeded by far the amount of endogenous SP bound originally in the brain extracts. 4. Among the lipids present in TLE, phosphatidylserine was able to bind and release SP in a pH dependent manner. 5. It is suggested that SP bound to phosphatidylserine is the storage form of SP in the brain. The mechanisms by which it is released are still unknown. The possibility that the SP-receptor is also a phospholipid is considered.

可以通过一种能提取所有脂质的方法，用氯仿 - 甲醇从脑匀浆中提取P物质（SP）。2. 在低pH值（2.0 - 3.0）下，SP可以从这种总脂质提取物（TLE）转移到水溶液中。3. 在较高pH值（5.5）时，SP可以从水相转移到有机相（氯仿：甲醇，2:1）中，并与该相中所含的TLE（先前已去除内源性SP）重新结合。TLE对SP的结合能力远远超过脑提取物中最初结合的内源性SP的量。4. 在TLE中存在的脂质中，磷脂酰丝氨酸能够以pH依赖的方式结合和释放SP。5. 有人提出，与磷脂酰丝氨酸结合的SP是脑中SP的储存形式。其释放的机制仍然未知。还考虑了SP受体也是一种磷脂的可能性。