College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, China.
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, China.
Sci Total Environ. 2017 Jan 1;575:87-96. doi: 10.1016/j.scitotenv.2016.10.003. Epub 2016 Oct 10.
It is very important to have a good understanding of the biological effects of nanoparticles (NPs) on marine diatoms. In this study, the physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum to titanium dioxide NPs (nano-TiO) and cerium oxide NPs (nano-CeO) were compared and evaluated using 96h growth tests in a batch-culture system. At 96h of exposure, the growth inhibition rate (IR, %) of P. tricornutum increased from 5.46 to 27.31% with increasing nano-TiO concentrations from 2.5 to 40mgL. The maximum IR of 49.59% occurred in 40mgL nano-TiO treatments at 48h of exposure. Growth of the diatom was increased in low nano-CeO treatments (≤5mgL), but was inhibited in high nano-CeO treatments (≥10mgL). Large aggregates of NPs were attached to the cells of P. tricornutum in 20 and 40mgL nano-TiO and nano-CeO treatments. In addition, the effective quantum yields (Φ) of P. tricornutum in 40mgL nano-TiO and nano-CeO treatments were 83.33 and 71.13% of that in the controls at 96h of exposure, respectively. Compared with that of the controls at 96h of exposure, chlorophyll a content, soluble sugar content, malondialdehyde (MDA) content, SOD and POD activities of P. tricornutum in 40mgL nano-TiO and nano-CeO treatments increased by 57.56, 142.97, 373.25, 698.76, 204.85% and 21.43, 89.41, 194.97, 340.05, 502.86%, while soluble protein content decreased by 70.38 and 28.64%, respectively. These findings will be helpful to understand the effect mechanisms of NPs on marine organisms.
研究纳米二氧化钛（nano-TiO）和纳米氧化铈（nano-CeO）对海洋硅藻的生物学效应，对于理解纳米颗粒（NPs）非常重要。在这项研究中，采用批式培养系统的 96h 生长试验，比较和评价了海洋硅藻三角褐指藻对 TiO2 NPs（nano-TiO）和 CeO2 NPs（nano-CeO）的生理生化响应。暴露 96h 后，随着 nano-TiO 浓度从 2.5 增加到 40mgL，P. tricornutum 的生长抑制率（IR，%）从 5.46%增加到 27.31%。在暴露 48h 时，浓度为 40mgL 的 nano-TiO 处理组的最大 IR 为 49.59%。低浓度的 nano-CeO（≤5mgL）处理促进了硅藻的生长，但高浓度的 nano-CeO（≥10mgL）处理则抑制了硅藻的生长。在 20 和 40mgL 的 nano-TiO 和 nano-CeO 处理中，大量的 NPs 聚集在 P. tricornutum 细胞上。此外，在暴露 96h 时，40mgL 的 nano-TiO 和 nano-CeO 处理组的 P. tricornutum 的有效量子产率（Φ）分别为对照组的 83.33%和 71.13%。与暴露 96h 的对照组相比，在 40mgL 的 nano-TiO 和 nano-CeO 处理组中，P. tricornutum 的叶绿素 a 含量、可溶性糖含量、丙二醛（MDA）含量、SOD 和 POD 活性分别增加了 57.56、142.97、373.25、698.76、204.85%和 21.43、89.41、194.97、340.05、502.86%，而可溶性蛋白含量分别下降了 70.38%和 28.64%。这些发现将有助于理解 NPs 对海洋生物的影响机制。
