Suto Ryu, Ishimoto Chikako, Chikyu Mikio, Aihara Yoshito, Matsumoto Toshimi, Uenishi Hirohide, Yasuda Tomoko, Fukumoto Yasuyuki, Waki Miyoko
Ibaraki Prefectural Livestock Research Center, 1234 Negoya, Ishioka, Ibaraki 315-0132, Japan.
Shizuoka Prefectural Research Institute of Animal Industry Swine & Poultry Research Center, 2780 Nishikata, Kikugawa, Shizuoka 439-0037 Japan.
Chemosphere. 2017 Jan;167:300-307. doi: 10.1016/j.chemosphere.2016.09.121. Epub 2016 Oct 11.
We investigated anammox with a focus on biofilm in 10 wastewater treatment plants (WWTPs) that use activated sludge treatment of swine wastewater. In three plants, we found red biofilms in aeration tanks or final sedimentation tanks. The biofilm had higher anammox 16S rRNA gene copy numbers (up to 1.35 × 10 copies/g-VSS) and higher anammox activity (up to 295 μmoL/g-ignition loss/h) than suspended solids in the same tank. Pyrosequencing analysis revealed that Planctomycetes accounted for up to 17.7% of total reads in the biofilm. Most of them were related to Candidatus Brocadia or Ca. Jettenia. The highest copy number and the highest proportion of Planctomycetes were comparable to those of enriched anammox sludge. Thus, swine WWTPs that use activated sludge treatment can fortuitously acquire anammox biofilm. Thus, concentrated anammox can be detected by focusing on red biofilm.
我们对10个采用活性污泥法处理猪废水的污水处理厂（WWTPs）中的厌氧氨氧化进行了研究，重点关注生物膜。在三个工厂中，我们在曝气池或最终沉淀池发现了红色生物膜。与同一池中悬浮固体相比，该生物膜具有更高的厌氧氨氧化16S rRNA基因拷贝数（高达1.35×10拷贝/g-VSS）和更高的厌氧氨氧化活性（高达295 μmoL/g-灼烧减量/h）。焦磷酸测序分析表明，浮霉菌门在生物膜总读数中占比高达17.7%。其中大多数与“暂定布罗卡德氏菌属”或“暂定杰氏菌属”相关。浮霉菌门的最高拷贝数和最高比例与富集的厌氧氨氧化污泥相当。因此，采用活性污泥处理的猪污水处理厂可能会偶然获得厌氧氨氧化生物膜。因此，通过关注红色生物膜可以检测到浓缩的厌氧氨氧化。
