Nie Xiao-Juan, Liu Wen-Min, Zhang Li
Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).
Med Sci Monit. 2016 Oct 12;22:3658-3665. doi: 10.12659/msm.896710.
BACKGROUND Cutaneous squamous cell carcinoma (cSCC) is the second most common type of non-melanoma skin cancer (NMSC) globally. The aims of this study were to further systematically clarify the potential association of rs833061 (-460 C>T) and rs1570360 (-1154 G>A), two SNPs of VEGF, with the risk of cSCC and the prognostic impacts on cSCC patients. MATERIAL AND METHODS This hospital-based case-control study analyzed peripheral venous blood collected from 100 cSCC patients and 124 healthy controls, and gathered personal information on patients. Genotypes of the VEGF gene -460C>T and -1154G>A polymorphism were detected using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. Different distributions of allele frequencies and genotype in the case and control group were measured, comparing different genotype differences in the survival of patients with cSCC. RESULTS Distributions of allele frequencies and genotype of -460 C>T in the case and control group were statistically different; the TT + CT genotype was significantly correlated with a decrease risk of cSCC (OR=0.36, 95% CI=0.21-0.63, P<0.001). There was no difference in the distribution of allele frequencies and genotype of -1154 G>A between control and case groups. For -1154460C>T, the CC genotype was an adverse factor, associated with a significant decrease in the survival status of cSCC patients (P<0.001). For VEGF-1154 G>A, the AA genotype was significantly correlated with the reduced overall survival in cSCC patients, with the mean survival time of 23.88 months (P=0.009). CONCLUSIONS The VEGF gene -460 C>T polymorphism and -1154 G>A polymorphism may serve as potential genetic markers for the risk and prognosis of cSCC.
背景 皮肤鳞状细胞癌（cSCC）是全球第二常见的非黑色素瘤皮肤癌（NMSC）类型。本研究的目的是进一步系统地阐明血管内皮生长因子（VEGF）的两个单核苷酸多态性（SNP），即rs833061（-460 C>T）和rs1570360（-1154 G>A）与cSCC风险的潜在关联以及对cSCC患者的预后影响。材料与方法 这项基于医院的病例对照研究分析了从100例cSCC患者和124名健康对照者采集的外周静脉血，并收集了患者的个人信息。采用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）方法检测VEGF基因-460C>T和-1154G>A多态性的基因型。测量病例组和对照组中等位基因频率和基因型的不同分布，比较cSCC患者生存中不同基因型差异。结果 病例组和对照组中-460 C>T的等位基因频率和基因型分布存在统计学差异；TT + CT基因型与cSCC风险降低显著相关（OR=0.36，95%可信区间[CI]=0.21-0.63，P<0.001）。对照组和病例组之间-1154 G>A的等位基因频率和基因型分布没有差异。对于-1154460C>T，CC基因型是一个不利因素，与cSCC患者生存状况显著下降相关（P<0.001）。对于VEGF-1154 G>A，AA基因型与cSCC患者总生存期缩短显著相关，平均生存时间为23.88个月（P=0.009）。结论 VEGF基因-460 C>T多态性和-1154 G>A多态性可能作为cSCC风险和预后的潜在遗传标志物。
