Debec Alain, Megraw Timothy L, Guichet Antoine
Institut Jacques Monod, UMP 7592-CNRS, Université Paris Diderot, Paris, France.
Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL, USA.
Methods Mol Biol. 2016;1478:333-351. doi: 10.1007/978-1-4939-6371-3_21.
Hundreds of Drosophila cell lines have been established in the labs of many researchers over the last decades and have been important tools for research. Although these cells often deviate from normal cell physiology and genetic composition, such systems nonetheless are powerful models for biochemical, cell biological, and genetics studies that are experimentally difficult in vivo. While published descriptions of cell line generation are available in the literature, how to generate new Drosophila cell lines can be challenging for beginners. Here, we describe a detailed, simple protocol to establish new Drosophila cell lines.
在过去几十年里，许多研究人员的实验室已建立了数百种果蝇细胞系，它们一直是重要的研究工具。尽管这些细胞常常偏离正常细胞生理学和基因组成，但对于体内实验困难的生物化学、细胞生物学和遗传学研究而言，这样的系统仍是强大的模型。虽然文献中有已发表的关于细胞系生成的描述，但对于初学者来说，如何生成新的果蝇细胞系可能具有挑战性。在此，我们描述一种详细、简单的建立新果蝇细胞系的方案。
