Adili Nezar, Melizi Mohamed, Belabbas Hadj
Department of Veterinary Medicine, Institute of Veterinary Sciences and Agricultural Sciences, University of Batna 1, Batna 05000, Algeria.
Department of Microbiology and Biochemistry, Faculty of Sciences, University of Mohamed BOUDIAF, M'sila 28000, Algeria.
Vet World. 2016 Sep;9(9):960-963. doi: 10.14202/vetworld.2016.960-963. Epub 2016 Sep 11.
This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells.
Blood samples were taken from the jugular vein; and the smears were confectioned on slides immediately after the blood collection and stained according to the May-Gründwald Giemsa method. For the morphometric study, three parameters were considered which are: The diameter, the circumference, and the surface of erythrocytes; and measurements were achieved using the OPTIKATM Vision Pro software. Statistical analysis was performed by both analysis of variances and Student's t analytical tests.
The recorded data showed that the three morphometric parameters of red blood cells are higher in dogs followed, respectively, by those of horses, cattle, and sheep, whereas, the goats have the lowest ones. In addition, the obtained results allowed us to propose new reference values for the circumference and the surface of erythrocyte in considered species.
This investigation permit concluding that from a drop of blood it is possible to characterize the different animal species, taking into account the diameter, the circumference, and the surface of erythrocytes.
本研究是在已发表的初步研究的延续背景下进行的；研究对象包括牛、羊、山羊、马和狗；主要目的是根据红细胞的形态测量参数揭示并制定动物物种鉴定标准。
从颈静脉采集血样；采血后立即在载玻片上制作涂片，并按照美-格吉姆萨法染色。对于形态测量研究，考虑了三个参数，即红细胞的直径、周长和表面积；使用OPTIKATM Vision Pro软件进行测量。通过方差分析和学生t检验进行统计分析。
记录的数据表明，狗的红细胞三个形态测量参数最高，其次分别是马、牛和羊，而山羊的最低。此外，所得结果使我们能够为所考虑物种的红细胞周长和表面积提出新的参考值。
本研究可以得出结论，考虑到红细胞的直径、周长和表面积，从一滴血中就有可能对不同动物物种进行特征描述。
