Bakonyi József, Seress Diána, Nagy Zoltán Á, Csorba Ildikó, Cséplő Mónika, Marton Tibor A, Martin Anke, Mészáros Klára
Plant Protection Institute, HUN-REN Centre for Agricultural Research, Herman Ottó Str. 15, 1022 Budapest, Hungary.
Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, 613 00 Brno, Czech Republic.
J Fungi (Basel). 2024 Feb 28;10(3):184. doi: 10.3390/jof10030184.
f. (Ptt), the causal agent of net form net blotch (NFNB) disease, is an important and widespread pathogen of barley. This study aimed to quantify and characterize the virulence of Ptt isolates collected from experimental fields of barley in Hungary. Infection responses across 20 barley differentials were obtained from seedling assays of 34 Ptt isolates collected from three Hungarian breeding stations between 2008 and 2018. Twenty-eight Ptt pathotypes were identified. Correspondence analysis followed by hierarchical clustering on the principal components and host-by-pathogen GGE biplots suggested a continuous range of virulence and an absence of specific isolate × barley differential interactions. The isolates were classified into four isolate groups (IG) using agglomerative hierarchical clustering. One IG could be distinguished from other IGs based on avirulence/virulence on one to five barley differentials. Several barley differentials expressed strong resistance against multiple Ptt isolates and may be useful in the development of NFNB-resistant barley cultivars in Hungary. Our results emphasize that the previously developed international barley differential set needs to be improved and adapted to the Hungarian Ptt population. This is the first report on the pathogenic variations of Ptt in Hungary.
f. （Ptt）是网斑病（NFNB）的致病因子，是大麦的一种重要且广泛存在的病原体。本研究旨在对从匈牙利大麦试验田中采集的Ptt分离株的毒力进行量化和特征分析。通过对2008年至2018年间从匈牙利三个育种站采集的34个Ptt分离株进行幼苗试验，获得了20个大麦鉴别品种的感染反应。鉴定出了28种Ptt致病型。对应分析，随后对主成分进行层次聚类以及寄主-病原体GGE双标图分析表明，毒力呈连续变化，且不存在特定分离株×大麦鉴别品种的相互作用。使用凝聚层次聚类将分离株分为四个分离株组（IG）。基于对一至五个大麦鉴别品种的无毒力/毒力情况，可将一个IG与其他IG区分开来。几个大麦鉴别品种对多个Ptt分离株表现出强抗性，可能有助于匈牙利培育抗NFNB的大麦品种。我们的结果强调，先前开发的国际大麦鉴别品种集需要改进并适应匈牙利的Ptt种群。这是关于匈牙利Ptt致病变异的首次报道。
