Thurik Florentine F, Lamain-de Ruiter Marije, Javadi Ahmad, Kwee Anneke, Woortmeijer Heleen, Page-Christiaens Godelieve C M L, Franx Arie, van der Schoot C Ellen, Koster Maria P H
Department of Experimental Immunohematology, Sanquin Research, Amsterdam and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
Division of Women and Baby, Department of Obstetrics, University Medical Centre Utrecht, Utrecht, The Netherlands.
Prenat Diagn. 2016 Dec;36(12):1104-1111. doi: 10.1002/pd.4940. Epub 2016 Nov 10.
To study associations of first trimester cell-free fetal DNA levels (in this paper referred to as cell-free placental DNA (cfpDNA) levels) and preeclampsia (PE), pregnancy-induced hypertension (PIH), gestational diabetes (GDM) and spontaneous preterm birth (sPB).
A nested case-control study was conducted in first trimester samples (gestational age 8 -13 weeks). A total of 226 cases and 301 controls were included. CfpDNA levels were quantified in male-bearing pregnancies using real-time DYS14-PCRs on DNA isolated from maternal serum. CfpDNA multiples of the median (MoMs) were calculated based on associations with patient characteristics (body mass index, parity, ethnicity and smoking). Associations between MoMs and adverse outcomes were studied.
Cell-free placental DNA levels were negatively associated with body mass index (β = -0.297, p < 0.001) and smoking (β = -0.163, p = 0.006). MoMs were lower in women who later developed PIH (n = 84, p = 0.009) or GDM (n = 56, p = 0.037). There was no association between cfpDNA MoMs and PE (n = 37, p = 0.15) or sPB (n = 49, p = 0.19). CfpDNA was positively correlated with pregnancy-associated plasma protein A (r = 0.426, p < 0.001) but not with placental growth factor (r = 0.059, p = 0.179).
Adjusted first trimester cfpDNA levels are associated with PIH and GDM but probably not with PE or sPB. © 2016 John Wiley & Sons, Ltd.
研究孕早期游离胎儿DNA水平（本文中称为游离胎盘DNA（cfpDNA）水平）与子痫前期（PE）、妊娠高血压（PIH）、妊娠期糖尿病（GDM）和自发性早产（sPB）之间的关联。
在孕早期样本（孕龄8 - 13周）中进行巢式病例对照研究。共纳入226例病例和301例对照。使用实时DYS14-PCR对从母血清中分离的DNA进行定量分析，以测定男性胎儿妊娠中的cfpDNA水平。根据与患者特征（体重指数、产次、种族和吸烟情况）的关联计算cfpDNA中位数倍数（MoMs）。研究MoMs与不良结局之间的关联。
游离胎盘DNA水平与体重指数呈负相关（β = -0.297，p < 0.001）和吸烟呈负相关（β = -0.163，p = 0.006）。后来发生PIH（n = 84，p = 0.009）或GDM（n = 56，p = 0.037）的女性MoMs较低。cfpDNA MoMs与PE（n = 37，p = 0.15）或sPB（n = 49，p = 0.19）之间无关联。cfpDNA与妊娠相关血浆蛋白A呈正相关（r = 0.426，p < 0.001），但与胎盘生长因子无相关性（r = 0.059，p = 0.179）。
校正后的孕早期cfpDNA水平与PIH和GDM相关，但可能与PE或sPB无关。© 2016约翰威立国际出版公司。
