Grün R, Grün M, Rolfs A, Kaffarnik H
Medizinische Klinik, Recklinghausen.
Z Gastroenterol. 1989 Jul;27(7):378-82.
Hormones especially estrogens have been suspected to induce liver cell tumours or hepatic focal nodular hyperplasia (FNH). In rats 6 months after portocaval anastomosis (PCA) the occurrence of FNH has been observed. Modified portocaval anastomosis (mPCA) does not lead to FNH. To test the concept of estrogen induced tumour formation we measured in both groups as well as in a shamoperated control group (SOP) the levels of estradiol (E2), estrone (E1) and of testosterone (T). In a further experiment the hormone levels were measured in rats with portocaval transposition (PCT), an operation which leads to high gonadal hormone production. In groups of 6 male rats each (280-300 g) either SOP, PCA, mPCA or PCT were performed. 30 days later the blood levels of E2, E1, and of T were measured by radioimmunoassay. In PCT-rats hormone levels were measured in the blood synchronously taken from the inferior vena cava (prehepatic) and from the heart (posthepatic), to get an information of the hepatic hormone degradation. After PCA the median level of E2 (77 pg/ml) and E1 (63 pg/ml) are significantly elevated when compared with SOP-rats (41 and 43 pg/ml). Equally after mPCA the E2 and E1 levels are significantly higher (61 and 70 pg/ml) than in controls rats. In contrast the concentrations of T are significantly reduced (PCA 0.03, mPCA 0, 10, SOP 1.0 ng/ml). PCA as well as mPCA result in a hyperestrogenic and hypoandrogenic status. When PCA and mPCA are compared, only the testosterone blood levels are significantly different.(ABSTRACT TRUNCATED AT 250 WORDS)
激素尤其是雌激素，一直被怀疑会诱发肝细胞肿瘤或肝局灶性结节性增生（FNH）。在大鼠门腔静脉吻合术（PCA）6个月后，已观察到FNH的发生。改良门腔静脉吻合术（mPCA）不会导致FNH。为了验证雌激素诱导肿瘤形成的概念，我们在这两组以及假手术对照组（SOP）中测量了雌二醇（E2）、雌酮（E1）和睾酮（T）的水平。在进一步的实验中，测量了门腔静脉转位（PCT）大鼠的激素水平，该手术会导致高性腺激素产生。每组6只雄性大鼠（体重280 - 300克），分别进行SOP、PCA、mPCA或PCT手术。30天后，通过放射免疫分析法测量E2、E1和T的血液水平。在PCT大鼠中，同步从下腔静脉（肝前）和心脏（肝后）采集血液测量激素水平，以了解肝脏对激素的降解情况。与SOP大鼠（分别为41和43 pg/ml）相比，PCA后E2（77 pg/ml）和E1（63 pg/ml）的中位数水平显著升高。同样，mPCA后E2和E1水平也显著高于对照大鼠（分别为61和70 pg/ml）。相比之下，T的浓度显著降低（PCA为0.03，mPCA为0.10，SOP为1.0 ng/ml）。PCA和mPCA都会导致高雌激素和低雄激素状态。当比较PCA和mPCA时，只有睾酮的血液水平有显著差异。（摘要截选至250字）
