Koczurkiewicz Paulina, Kowolik Ewa, Podolak Irma, Wnuk Dawid, Piska Kamil, Łabędź-Masłowska Anna, Wójcik-Pszczoła Katarzyna, Pękala Elżbieta, Czyż Jarosław, Michalik Marta
Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
Department of Pharmacognosy, Faculty of Pharmacy Medical College, Jagiellonian University, Kraków, Poland.
Planta Med. 2016 Dec;82(18):1546-1552. doi: 10.1055/s-0042-117537. Epub 2016 Oct 13.
Triterpene saponins are secondary metabolites typical for higher plants. They possess a wide range of pharmaceutical and biological activities. These include anti-inflammatory, vasoprotective, expectorant, and antitumor properties. In particular, the ability of saponins to enhance the cytotoxicity of chemotherapeutic drugs has opened new perspectives for their application in combined cancer chemotherapy. In this study, the biological activity of the saponin fraction isolated from (denoted as CIL-1/2) was evaluated to assess its chemosensitizing activity in prostate cancer cell lines (DU-145, PC-3). No cytotoxic or cytostatic effect of the CIL-1/2 fraction administered at the concentration of 0.5 µg/mL was observed. In contrast, cocktails of CIL-1/2 and mitoxantrone (a drug commonly used in prostate cancer therapy) exerted synergistic cytostatic and proapoptotic effects. Furthermore, the synergy of proapoptotic activities of the analyzed cocktails is accompanied by their synergistic effects on prostate cancer cell movement and invasiveness. The significantly weaker impact of this cocktail on normal prostate cells additionally adds to the significance of our data and confirms that the CIL-1/2 fraction might be considered a potent adjuvant for prostate cancer chemotherapy.
三萜皂苷是高等植物特有的次生代谢产物。它们具有广泛的药理和生物学活性。这些活性包括抗炎、血管保护、祛痰和抗肿瘤特性。特别是，皂苷增强化疗药物细胞毒性的能力为其在联合癌症化疗中的应用开辟了新的前景。在本研究中，对从[具体来源未给出]分离得到的皂苷组分（记为CIL-1/2）的生物活性进行了评估，以评估其在前列腺癌细胞系（DU-145、PC-3）中的化学增敏活性。未观察到以0.5μg/mL浓度给药的CIL-1/2组分具有细胞毒性或细胞抑制作用。相反，CIL-1/2与米托蒽醌（一种常用于前列腺癌治疗的药物）的混合物发挥了协同的细胞抑制和促凋亡作用。此外，所分析混合物的促凋亡活性协同作用伴随着它们对前列腺癌细胞运动和侵袭的协同作用。该混合物对正常前列腺细胞的影响明显较弱，这进一步增加了我们数据的重要性，并证实CIL-1/2组分可被视为前列腺癌化疗的有效佐剂。
