5-氟尿嘧啶耐药结肠癌细胞中自噬的减少
Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells.
作者信息
Yao Cheng Wen, Kang Kyoung Ah, Piao Mei Jing, Ryu Yea Seong, Fernando Pattage Madushan Dilhara Jayatissa, Oh Min Chang, Park Jeong Eon, Shilnikova Kristina, Na Soo-Young, Jeong Seung Uk, Boo Sun-Jin, Hyun Jin Won
机构信息
School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
School of Medicine No.2, Jeju National University, Jeju 63241, Republic of Korea.
出版信息
Biomol Ther (Seoul). 2017 May 1;25(3):315-320. doi: 10.4062/biomolther.2016.069.
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
我们研究了自噬在SNUC5/5-FUR（5-氟尿嘧啶（5-FU）耐药的SNUC5结肠癌细胞）中的作用。通过光学显微镜、共聚焦显微镜以及吖啶橙染色后的流式细胞术检测发现，SNUC5/5-FUR细胞表现出自噬水平较低，并且绿色荧光蛋白标记的微管相关蛋白轻链3（GFP-LC3）斑点水平降低。此外，SNUC5/5-FUR细胞中关键自噬蛋白如自噬相关蛋白5（Atg5）、Beclin-1和LC3-II的表达以及自噬通量均减少。虽然活性氧（ROS）的产生在SNUC5/5-FUR细胞中显著升高，但用ROS抑制剂N-乙酰半胱氨酸处理后进一步降低了自噬水平。综上所述，这些结果表明自噬减少与SNUC5结肠癌细胞的5-FU耐药有关。
Zotero 插件