Dąbrowska G, Hrynkiewicz K, Trejgell A, Baum C
a Department of Genetics, Faculty of Biology and Environment Protection , Nicolaus Copernicus University , Toruń , Poland.
b Department of Microbiology, Faculty of Biology and Environment Protection , Nicolaus Copernicus University , Toruń , Poland.
Int J Phytoremediation. 2017 Jul 3;19(7):597-604. doi: 10.1080/15226514.2016.1244157.
The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium. In the pot experiment, the strains were used for single Ba, Pf, Va or combined Ba + Pf, Ba + Va, Pf + Va, and Ba + Pf + Va inoculation of B. napus growing in contaminated soil from alluvial deposits. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it. However, single inoculation with Ba significantly increased the chlorophyll content and K concentration in the leaves. The inoculation of rapeseed with Ba and Va strains was indicated to be the most promising combination for phytoextraction of Cd and Zn from contaminated soil. Combined inoculation with Pf+Va and Pf + Ba+Va significantly decreased the concentration of heavy metals in the roots of rapeseed. We conclude that suitable combinations of PGPR can control the metal uptake of B. napus, selectively increasing either metal extraction or metal stabilization in the rhizosphere and offering promising applications in soil remediation.
预先选择了测试菌株拟杆菌属细菌（Ba）、荧光假单胞菌（Pf）和贪铜菌属（Va），因为它们在培养基中镉、铜、铅和锌浓度增加的情况下具有促进油菜籽体外生长的能力。在盆栽试验中，这些菌株被用于对生长在冲积沉积物污染土壤中的甘蓝型油菜进行单一的Ba、Pf、Va接种，或组合的Ba + Pf、Ba + Va、Pf + Va以及Ba + Pf + Va接种。在体外观察到了细菌菌株对植物生长的积极影响，但在污染土壤中未得到实地证实，在污染土壤中，测试菌株抑制了生物量的产生，而不是刺激其生长。然而，单独接种Ba显著增加了叶片中的叶绿素含量和钾浓度。接种Ba和Va菌株对从污染土壤中植物提取镉和锌而言是最有前景的组合。Pf+Va以及Pf + Ba+Va的组合接种显著降低了油菜籽根部的重金属浓度。我们得出结论，合适的植物生长促进根际细菌组合可以控制甘蓝型油菜对金属的吸收，有选择地增加根际中的金属提取或金属稳定，并在土壤修复中具有广阔的应用前景。
