Michael Karen, No David, Dankoff Jennifer, Lee Kyoyi, Lara-Crawford Elisabeth, Roberts Marilyn C
FEMS Microbiol Lett. 2016 Nov 1;363(21). doi: 10.1093/femsle/fnw236.
Clostridium difficile is both a hospital and community-acquired pathogen. The current study determined if C. difficile could be cultured from clinical laundry facility surfaces. A total of 240 surface samples were collected from dirty areas (n = 120), which handle soiled clinical linens, and from clean areas (n = 120), which process and fold the clean linens, within the University of Washington Consolidated Laundry facility in 2015. Sampling was done four times over the course of 1 year. The dirty area was significantly more contaminated than the clean area (21% vs 2%, P < 0.001). Clostridium difficile isolates were genetically characterized using multilocus sequence typing and PCR for the detection of genes encoding toxin A and toxin B. The MLST types 1, 2, 3, 15, 26, 34, 35, 39, 42, 43, 44, 53, 63 and 284 were identified and have previously been found in both clinical and community settings. Toxin positive isolates were identified in both the dirty (n = 16/25) and clean areas (n = 2/2). Seasonal variation was observed with 40% of the 27 isolates cultured in April 2015. The study suggests that soiled clinical linens may be a source of C. difficile surface contamination.
艰难梭菌既是一种医院内获得性病原体，也是一种社区获得性病原体。当前研究确定了能否从临床洗衣设施表面培养出艰难梭菌。2015年，在华盛顿大学综合洗衣设施内，共从处理脏污临床织物的脏污区域（n = 120）和处理并折叠干净织物的清洁区域（n = 120）采集了240个表面样本。在1年的时间里进行了4次采样。脏污区域的污染程度明显高于清洁区域（21%对2%，P < 0.001）。使用多位点序列分型和PCR对艰难梭菌分离株进行基因特征分析，以检测编码毒素A和毒素B的基因。鉴定出了多位点序列分型类型1、2、3、15、26、34、35、39、42、43、44、53、63和284，这些类型此前在临床和社区环境中均有发现。在脏污区域（n = 16/25）和清洁区域（n = 2/2）均鉴定出了毒素阳性分离株。观察到了季节性变化，2015年4月培养的27株分离株中有40%出现这种情况。该研究表明，脏污的临床织物可能是艰难梭菌表面污染的一个来源。
