Liu Yanxia, Wang Lin, Wang Bingping, Yue Meng, Cheng Yufeng
Department of Radiation Oncology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China; Department of Oncology, Shengli Oil Central Hospital, Dongying, Shandong 257034, China.
Department of Radiation Oncology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.
Dis Markers. 2016;2016:2619828. doi: 10.1155/2016/2619828. Epub 2016 Sep 25.
Colon cancer is the third and second most common cancer form in men and women worldwide. It is generally accepted that colon cancer mainly results from diet. The aim of this study was to identify core pathways which elucidated the molecular mechanisms in colon cancer. The microarray data of E-GEOD-44861 was downloaded from ArrayExpress database. All human pathways were obtained from Kyoto Encyclopedia of Genes and Genomes database. In total, 135 differential expressed genes (DEG) were identified using Linear Models for Microarray Data package. Differential pathways were identified with the method of attractor after overlapping with DEG. Pathway cross talk network (PCN) was constructed by combining protein-protein interactions and differential pathways. Cross talks of all pathways were obtained in PCN. There were 65 pathways with RankProd (RP) values < 0.05 and 16 pathways with Impact Factors (IF) values > 100. Five pathways were satisfied with value < 0.05, RP values < 0.05, and IF values > 100, which were considered to be the most important pathways in colon cancer. In conclusion, the five pathways were identified in the center status of colon cancer, which may contribute to understanding the mechanism and development of colon cancer.
结肠癌是全球男性和女性中第三和第二常见的癌症形式。人们普遍认为结肠癌主要由饮食引起。本研究的目的是确定阐明结肠癌分子机制的核心通路。从ArrayExpress数据库下载了E-GEOD-44861的微阵列数据。所有人类通路均从京都基因与基因组百科全书数据库获得。使用微阵列数据线性模型软件包共鉴定出135个差异表达基因（DEG）。通过与DEG重叠后使用吸引子方法鉴定差异通路。通过结合蛋白质-蛋白质相互作用和差异通路构建通路串扰网络（PCN）。在PCN中获得所有通路的串扰情况。有65条通路的秩乘积（RP）值<0.05，16条通路的影响因子（IF）值>100。有五条通路满足 值<0.05、RP值<0.05且IF值>100，这些通路被认为是结肠癌中最重要的通路。总之，这五条通路在结肠癌中处于核心地位，可能有助于理解结肠癌的机制和发展。
