Diao Xiufang, Liu Aijuan
Department of Respiratory Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Department of Cardiology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Exp Ther Med. 2018 Feb;15(2):1520-1524. doi: 10.3892/etm.2017.5563. Epub 2017 Nov 24.
Ischemic stroke is a leading cause of mortality and disability around the world. It is an important task to identify dysregulated pathways which infer molecular and functional insights existing in high-throughput experimental data. Gene expression profile of E-GEOD-16561 was collected. Pathways were obtained from the database of Kyoto Encyclopedia of Genes and Genomes and Retrieval of Interacting Genes was used to download protein-protein interaction sets. Attractor and crosstalk approaches were applied to screen dysregulated pathways. A total of 20 differentially expressed genes were identified in ischemic stroke. Thirty-nine significant differential pathways were identified according to P<0.01 and 28 pathways were identified with RP<0.01 and 17 pathways were identified with impact factor >250. On the basis of the three criteria, 11 significant dysfunctional pathways were identified. Among them, Epstein-Barr virus infection was the most significant differential pathway. In conclusion, with the method based on attractor and crosstalk, significantly dysfunctional pathways were identified. These pathways are expected to provide molecular mechanism of ischemic stroke and represents a novel potential therapeutic target for ischemic stroke treatment.
缺血性中风是全球范围内导致死亡和残疾的主要原因。识别失调的信号通路是一项重要任务，这些通路能从高通量实验数据中推断出分子和功能方面的见解。收集了E-GEOD-16561的基因表达谱。信号通路来自京都基因与基因组百科全书数据库，并使用相互作用基因检索来下载蛋白质-蛋白质相互作用集。采用吸引子和串扰方法筛选失调的信号通路。在缺血性中风中总共鉴定出20个差异表达基因。根据P<0.01鉴定出39条显著差异信号通路，根据RP<0.01鉴定出28条信号通路，根据影响因子>250鉴定出17条信号通路。基于这三个标准，鉴定出11条显著功能失调的信号通路。其中，爱泼斯坦-巴尔病毒感染是最显著的差异信号通路。总之，通过基于吸引子和串扰的方法，鉴定出了显著功能失调的信号通路。这些信号通路有望为缺血性中风提供分子机制，并代表缺血性中风治疗的一种新的潜在治疗靶点。
