Kobayashi Mito, Kawase Tomoyuki, Okuda Kazuhiro, Wolff Larry F, Yoshie Hiromasa
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
Int J Implant Dent. 2015 Dec;1(1):31. doi: 10.1186/s40729-015-0032-0. Epub 2015 Nov 27.
Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios.
PRP, PRF, and platelet-poor plasma (PPP) were prepared from the peripheral blood of healthy donors. PRF preparations were squeezed or homogenized to produce exudate (PRFexu) or extract (PRFext), respectively. Concentrations of the angiogenic factors and their bioactivities were determined using ELISA kits, a scratch assay using endothelial cells and a chicken chorioallantoic membrane (CAM) assay.
In PRP and PRF preparations, both VEGF and PDGF-BB were significantly more concentrated than PPP. In the scratch assay, PRFexu and PRFext were the most effective for wound closure. In the CAM assay, PRF membranes were the most effective for neovascularization.
It is suggested that PRF preparations efficiently preserve the angiogenic factors and function not only as a scaffolding material but as a reservoir of angiogenic factors in wound healing.
富含血小板纤维蛋白（PRF）是富含血小板血浆（PRP）的衍生物，主要由纤维蛋白网络组成，在临床和临床前动物研究中越来越多地证明其在伤口愈合中有效。然而，人们仍然担心在通过缓慢凝血过程制备PRF期间，主要生长因子可能会大量流失。为了解决这一问题，我们通过基于体积比的程序标准化比较了PRF和PRP的血管生成潜力。
从健康供体的外周血中制备PRP、PRF和贫血小板血浆（PPP）。分别对PRF制剂进行挤压或匀浆，以产生渗出液（PRFexu）或提取物（PRFext）。使用酶联免疫吸附测定试剂盒、内皮细胞划痕试验和鸡胚绒毛尿囊膜（CAM）试验测定血管生成因子的浓度及其生物活性。
在PRP和PRF制剂中，VEGF和PDGF-BB的浓度均显著高于PPP。在划痕试验中，PRFexu和PRFext对伤口闭合最有效。在CAM试验中，PRF膜对新血管形成最有效。
提示PRF制剂能有效保留血管生成因子，不仅作为支架材料，而且作为伤口愈合中血管生成因子的储存库发挥作用。
