Samie Ghahfarokhi Mahshid, Reiisi Somayeh, Zamanzadeh Zahra, Abkar Morteza
Department of Genetics, Faculty of Biological Science and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
Iran J Public Health. 2023 May;52(5):1048-1060. doi: 10.18502/ijph.v52i5.12723.
miRNAs are small non-coding RNAs; regulate gene expression using RNA degradation or translation repression. Dysregulation of miRNAs is involved in the initiation and progression of many cancers. We aimed to determine the relationship between miR-5571-5p expression and clinical factors and regulatory mechanisms in breast cancer.
Histopathologic sections approximately with 25 microns thick from FFPE tissues were achievement of Al-Zahra Hospital (Isfahan, Iran) in 2020-2021 years by Pathologist. miR-5571-5p expression, determined using real-time PCR. For miRNA target genes prediction, integrated miRNA target prediction tools, were used. Gene ontology and KEGG pathway analysis were accomplished to identify the biological function. A PPI network was constructed to display key target genes. For hub genes validation, GEPIA databases were used.
miR-5571-5p was upregulated in breast tumor tissues, and its increase was significantly related to a poor prognosis in breast cancer (<0.0001). At first, 324 target genes were predicted, and then 110 genes with a decrease in expression were selected. GO analysis showed that genes were mainly enriched in the regulation of the ERBB2 and EGFR signaling pathway. KEGG pathway analysis suggested that downregulated genes were enriched in glioma, the ErbB signaling pathway, and breast cancer. Finally, the ten hub genes (, , , , , , , , , and ) were detected from the PPI network.
miR-5571-5p up-regulation is associated with breast cancer progression and worse survival. The current study identified ten genes associated with breast cancer, which might help to provide candidate targets for the treatment.
微小RNA（miRNAs）是一类小的非编码RNA，通过RNA降解或翻译抑制来调节基因表达。miRNAs的失调与多种癌症的发生和发展有关。我们旨在确定miR-5571-5p表达与乳腺癌临床因素及调控机制之间的关系。
2020 - 2021年，病理学家从阿尔-扎赫拉医院（伊朗伊斯法罕）获取了厚度约为25微米的福尔马林固定石蜡包埋（FFPE）组织的组织病理学切片。使用实时定量PCR测定miR-5571-5p的表达。使用综合的miRNA靶标预测工具进行miRNA靶基因预测。进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析以确定生物学功能。构建蛋白质-蛋白质相互作用（PPI）网络以展示关键靶基因。使用GEPIA数据库对枢纽基因进行验证。
miR-5571-5p在乳腺肿瘤组织中上调，其升高与乳腺癌的不良预后显著相关（<0.0001）。首先，预测了324个靶基因，然后选择了110个表达下调的基因。GO分析表明，这些基因主要富集于ERBB2和EGFR信号通路的调控。KEGG通路分析表明，下调的基因在胶质瘤、ErbB信号通路和乳腺癌中富集。最后从PPI网络中检测到10个枢纽基因（此处原文未列出具体基因名称）。
miR-5571-5p上调与乳腺癌进展和较差的生存率相关。本研究鉴定了10个与乳腺癌相关的基因，这可能有助于为治疗提供候选靶点。
