Guillard O, Lauwerys R
Unité de Toxicologie Industrielle et Médecine du Travail, Université Catholique de Louvain, Brussels, Belgium.
Biochem Pharmacol. 1989 Sep 1;38(17):2819-23. doi: 10.1016/0006-2952(89)90436-x.
A method based on the use of the luminogenic substance, luminol, was developed for measuring the respiratory burst induced by phorbolmyristate acetate in 4 microliters of human whole blood. In vitro, the three cations, Hg2+, Pb2+ and Cd2+, inhibit the peak luminescence response at concentrations higher than those usually found in blood of subjects exposed to these metals. Cadmium metallothionein, however, in a concentration range similar to that found in whole blood of workers exposed to cadmium strikingly reduces the respiratory oxidative burst. In workers exposed to inorganic lead or mercury vapour, no reduction of the chemiluminescence response of whole blood was observed while a slight but statistically significant reduction was found in whole blood from cadmium exposed workers. If a similar effect occurs in lung macrophages, it might decrease the respiratory tract resistance to infection.
开发了一种基于使用发光物质鲁米诺的方法，用于测量4微升人全血中佛波酯肉豆蔻酸酯诱导的呼吸爆发。在体外，Hg2 +、Pb2 +和Cd2 +这三种阳离子在高于接触这些金属的受试者血液中通常浓度时会抑制峰值发光反应。然而，镉金属硫蛋白在与接触镉的工人全血中发现的浓度范围相似时，会显著降低呼吸氧化爆发。在接触无机铅或汞蒸气的工人中，未观察到全血化学发光反应的降低，而在接触镉的工人全血中发现了轻微但具有统计学意义的降低。如果在肺巨噬细胞中发生类似作用，可能会降低呼吸道对感染的抵抗力。
