Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea.
Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea.
Nutrients. 2016 Oct 14;8(10):640. doi: 10.3390/nu8100640.
Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% (/) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.
绿茶（GT）具有多种健康功效，包括抗肥胖特性。然而，其作用的多种分子机制尚未完全确定。本研究旨在通过基于 RNA-seq 图谱分析其代谢和转录反应来阐明 GT 的抗肥胖作用。C57BL/6J 小鼠喂食正常饮食、高脂肪（60%能量来自脂肪）饮食或高脂肪+0.25%（/）GT 饮食 12 周。GT 提取物可改善肥胖、肝脂肪变性、血脂异常和胰岛素抵抗。GT 补充可通过增加能量消耗和减少脂肪量来减少肥胖小鼠的体重增加，而不是通过高脂肪饮食。附睾白色脂肪组织（eWAT）的转录组谱表明，GT 增强了对支链氨基酸（BCAA）降解以及 AMP 激活蛋白激酶（AMPK）信号的转录反应，这表明增强了能量稳态。我们的研究结果为 GT 预防肥胖及其合并症提供了一些重要的见解。我们表明，GT 提取物通过不仅调节脂肪和葡萄糖代谢，而且还通过脂肪组织中 BCAA 降解调节氨基酸代谢，有助于调节全身性代谢稳态。在 DIO 小鼠中。
