Szeto Yim Tong, Wong Kam Shing, Han Andrea, Pak Sok Cheon, Kalle Wouter
Department of Applied Science, Hong Kong Institute of Vocational Education (Shatin), Vocational Training Council, Hong Kong.
Department of Pathology, Yan Chai Hospital, Kowloon, Hong Kong.
J Intercult Ethnopharmacol. 2016 Jun 25;5(4):331-334. doi: 10.5455/jice.20160521021323. eCollection 2016 Sep-Dec.
The aim of this clinical study is to provide scientific evidence for supporting traditional Chinese application and usage to the patients. For this purpose, we tested the ability if Panax ginseng extract to lower oxidative damage to nuclear DNA in human lymphocytes by comparing the effect of cooked Chinese turnip on this effect.
Seven healthy subjects (4 males and 3 females from 37 to 60 years) participated two occasions which were at least 2 weeks apart. About 2 mL of fasting blood sample for baseline measurement was taken on arrival. They were requested to ingest the content of 5 ginseng capsules in 200 mL water. The subject remained fasting for 2 h until the second blood sample taken. In the other occasion, the experiment was repeated except a piece of cooked turnip (10 g) was taken with the ginseng extract. The two occasions could be interchanged. Comet assay was performed on two specimens on the same day for the evaluation of lymphocytic DNA damage with or without oxidative stress.
For the group with ginseng supplementation, there was a significant decrease in comet score for hydrogen peroxide (HO) treatment over the 2-h period while no change in DNA damage for unstressed sample. For the group with ginseng together with turnip supplementation, there was no significant difference in comet score for both HO treatment and phosphate-buffered saline treatment. Ginseng extract could reduce DNA damage mediated by HO effectively, but this protection effect was antagonized by the ingestion of cooked turnip at the same time.
In the current study, commercial ginseng extract was used for supplementing volunteers. Ginseng extract could protect DNA from oxidative stress while turnip diminished the protection.
本临床研究的目的是为向患者提供支持中药应用和用法的科学证据。为此，我们通过比较熟白萝卜对人参提取物降低人淋巴细胞核DNA氧化损伤作用的影响，来测试人参提取物的这种能力。
7名健康受试者（4名男性和3名女性，年龄37至60岁）分两次参与研究，两次间隔至少2周。到达时采集约2 mL空腹血样用于基线测量。要求他们将5粒人参胶囊的内容物溶于200 mL水中服用。受试者保持禁食2小时，直到采集第二份血样。在另一次实验中，除了与人参提取物一起食用一块熟白萝卜（10 g）外，实验过程重复进行。这两次实验可以互换。同一天对两份样本进行彗星试验，以评估有无氧化应激情况下的淋巴细胞DNA损伤。
对于补充人参的组，在2小时内过氧化氢（HO）处理后的彗星评分显著降低，而未受应激样本的DNA损伤无变化。对于同时补充人参和白萝卜的组，HO处理和磷酸盐缓冲盐水处理后的彗星评分均无显著差异。人参提取物可有效减少HO介导的DNA损伤，但同时摄入熟白萝卜会拮抗这种保护作用。
在本研究中，使用市售人参提取物对志愿者进行补充。人参提取物可保护DNA免受氧化应激，而白萝卜会削弱这种保护作用。
