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自体脂肪来源干细胞或基质血管成分局部动脉内输注的体内微循环反应

Microcirculatory Response In Vivo on Local Intraarterial Infusion of Autogenic Adipose-derived Stem Cells or Stromal Vascular Fraction.

作者信息

Wang Wei Z

机构信息

Division of Plastic Surgery, Department of Surgery, School of Medicine, University of Nevada, Las Vegas, Nev.

出版信息

Plast Reconstr Surg Glob Open. 2016 Sep 28;4(9):e1067. doi: 10.1097/GOX.0000000000001067. eCollection 2016 Sep.

Abstract

UNLABELLED

Both adipose-derived stem cells (ASCs) and stromal vascular fraction (SVF) have been demonstrated to have regenerative properties with therapeutic potential for numerous diseases through local or topical applications. However, it is unclear whether ASC or SVF can be delivered systemically through an intra-arterial infusion. The purpose of this study was to examine the microcirculatory response in vivo on local intraarterial infusion of autogenic ASCs or SVF in a vascular pedicle isolated rat cremaster microcirculation model.

MATERIALS AND METHODS

Fat tissue was surgically harvested from the flanks of male Sprague-Dawley rats (n = 12) and processed for SVF isolation. Some SVF samples were cultured for 24 hours for ASC purification. The autogenic SVF (1 × 10) cells (n = 6) or purified ASC (1 × 10) cells (n = 6) cells were infused into the microcirculation of cremaster muscle at a speed of 0.05 mL/min through the cannulation of femoral artery. As this is a vascular pedicle isolated preparation, the infused SVF or ASC cells went nowhere but the cremaster muscle. The video image of the microcirculation was monitored in real time during infusion.

RESULTS

Arteriole diameter was measured as A1 (100-160 µm), A2 (40-80 µm), and A3/A4 (10-30 µm). Capillary perfusion was quantified in 18 capillary fields of each muscle. There was a significant increase in the diameter of terminal arterioles ( = 0.049) and the capillary density ( = 0.02) after ASC intraarterial infusion. However, a significant cell aggregation, embolisms, and arterial obstruction were observed in the microcirculation in every case during SVF infusion.

CONCLUSIONS

Intraarterial infusion is an appropriate route for the delivery of autogenic ASCs, but not of SVF. SVF-induced microembolisms were the reason for narrowing or blocking the lumen of terminal arterioles, resulting in no flow in the corresponding capillaries.

摘要

未标记

脂肪来源干细胞(ASC)和基质血管成分(SVF)均已被证明具有再生特性,通过局部或局部应用对多种疾病具有治疗潜力。然而,尚不清楚ASC或SVF是否可以通过动脉内输注进行全身递送。本研究的目的是在血管蒂分离的大鼠提睾肌微循环模型中,研究自体ASC或SVF局部动脉内输注后的体内微循环反应。

材料与方法

从雄性Sprague-Dawley大鼠(n = 12)的侧腹手术采集脂肪组织,并进行SVF分离处理。一些SVF样本培养24小时以纯化ASC。将自体SVF(1×10)个细胞(n = 6)或纯化的ASC(1×10)个细胞(n = 6)通过股动脉插管以0.05 mL/min的速度注入提睾肌的微循环中。由于这是一个血管蒂分离的制剂,注入的SVF或ASC细胞除了提睾肌外无处可去。在输注过程中实时监测微循环的视频图像。

结果

小动脉直径测量为A1(100 - 160 µm)、A2(40 - 80 µm)和A3/A4(10 - 30 µm)。在每块肌肉的18个毛细血管区域中对毛细血管灌注进行定量。ASC动脉内输注后,终末小动脉直径(P = 0.049)和毛细血管密度(P = 0.02)显著增加。然而,在SVF输注过程中,每例均在微循环中观察到明显的细胞聚集、栓塞和动脉阻塞。

结论

动脉内输注是自体ASC递送的合适途径,但不是SVF的合适途径。SVF诱导的微栓塞是终末小动脉管腔狭窄或阻塞的原因,导致相应毛细血管无血流。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e21/5055030/0e3669ec0d12/gox-4-e1067-g001.jpg

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