Kim Hye Jin, Yoon Hae Min, Kwon Oran, Lee Won Jun
Department of Kinesiology and Sports Studies, College of Science and Industry Convergence, Ewha Womans University, Seoul Republic of Korea.
Department of Nutritional Science and Food Management, College of Science and Industry Convergence, Ewha Womans University, Seoul Republic of Korea.
J Exerc Nutrition Biochem. 2016 Sep;20(3):32-38. doi: 10.20463/jenb.2016.09.20.3.5. Epub 2016 Sep 30.
Pueraria lobata/rehmannia glutinosa (PR) and exercise have been receiving a lot of attention from postmenopausal women, as a result of the side effects of estrogen replacement therapy. However, the effects of PR and exercise on fatty acid transporters (FATPs), which play essential role in fatty acid transport, have not been studied. In this study, we evaluated the effects of PR and aerobic exercise on FATP1, FABPpm and FAT/CD36 expression in ovariectomized rat skeletal muscles.
Sixty rats were randomly divided into 6 groups: (1)HSV; high fat diet (HFD)+sedentary+vehicle, (2)HSP; HFD+sedentary+PR, (3)HSH; HFD+sedentary+17β-estradiol, (4)HEV; HFD+exercise+vehicle, (5) HEP; HFD+exercise+PR, (6)HEH; HFD+exercise+17β-estradiol. Exercise consisted of treadmill exercise (1-4th week: 15 m/min for 30 min, 5-8th week: 18 m/min for 40 min, 5 times/week).
Exercise does not alter FATP1 and FAT/CD36 gene levels in soleus and plantaris muscles. In contrast, exercise had main effect on up-regulation of FABPpm mRNA expression in both muscles. However, FABPpm level was not increased by exercise combined with treatments, indicative of no additive effects of PR or hormone on FABPpm gene expression. On the other hand, immunohistochemistry result showed that translocation of FATPs proteins to plasma membrane were higher in PR, exercise groups, and exercise combined with PR groups in both muscles.
These result showed that aerobic exercise and PR may help increase fat-oxidation through the induction of FABPpm, a muscle specific transporter, in OVX rat skeletal muscles. In addition, FABPpm expression is possibly regulated post-transcriptionally in exercise, or pre-translationally in PR.
由于雌激素替代疗法的副作用，葛根/熟地黄（PR）和运动受到了绝经后女性的广泛关注。然而，PR和运动对在脂肪酸转运中起关键作用的脂肪酸转运蛋白（FATP）的影响尚未得到研究。在本研究中，我们评估了PR和有氧运动对去卵巢大鼠骨骼肌中FATP1、FABPpm和FAT/CD36表达的影响。
60只大鼠随机分为6组：（1）HSV；高脂饮食（HFD）+久坐不动+赋形剂，（2）HSP；HFD+久坐不动+PR，（3）HSH；HFD+久坐不动+17β-雌二醇，（4）HEV；HFD+运动+赋形剂，（5）HEP；HFD+运动+PR，（6）HEH；HFD+运动+17β-雌二醇。运动包括跑步机运动（第1 - 4周：15米/分钟，持续30分钟；第5 - 8周：18米/分钟，持续40分钟，每周5次）。
运动并未改变比目鱼肌和跖肌中FATP1和FAT/CD36基因水平。相反，运动对这两种肌肉中FABPpm mRNA表达的上调有主要影响。然而，运动联合治疗并未增加FABPpm水平，表明PR或激素对FABPpm基因表达无累加效应。另一方面，免疫组化结果显示，在两种肌肉中，PR组、运动组以及运动联合PR组中FATP蛋白向质膜的转位更高。
这些结果表明，有氧运动和PR可能通过诱导FABPpm（一种肌肉特异性转运蛋白）来帮助增加去卵巢大鼠骨骼肌中的脂肪氧化。此外，FABPpm的表达在运动中可能在转录后受到调节，或在PR中在翻译前受到调节。
