Faculty of Pharmacy, Department of Toxicology, Mersin University, 33169, Mersin, Turkey.
Faculty of Pharmacy, Department of Toxicology, Gazi University, 06330, Ankara, Turkey.
Contact Dermatitis. 2017 Feb;76(2):89-95. doi: 10.1111/cod.12702. Epub 2016 Oct 19.
Henna has a very low allergic potential, and severe allergenic contact dermatitis is mainly caused by p-phenylenediamine (PPD), which is added to temporary black 'henna tattoos', and potentially also by some heavy metals.
To determine the presence of, and quantify, Lawsone, PPD and heavy metal contaminants (cobalt, nickel, lead, and chromium) in commercial temporary black henna tattoo mixtures (n = 25) sold in Turkey.
Lawsone and PPD concentrations were analysed with high-performance liquid chromatography, and heavy metal quantification was performed with inductively coupled plasma mass spectrometry.
PPD was found in all 25 black henna tattoo samples purchased from tattoo shops; levels varied between 3.37% and 51.6%. Lawsone was detected (0.002-88.2%) in 21 of the 25 temporary black henna tattoo samples analysed. Heavy metal contaminant levels were 0.44-3.11 ppm for Co, 1.13-2.20 ppm for Ni, 1.59-17.7 ppm for Pb, and 35.0-76.9 ppm for Cr.
Our results suggest that commercial temporary black henna mixtures containing PPD levels up to 51.6% pose a risk of contact sensitization and severe allergic contact dermatitis among users. It is important to identify both the additives and metallic contaminants of black henna tattoo products; the significance of metal contaminants has still to be assessed.
指甲花的过敏可能性非常低，而严重的过敏性接触性皮炎主要是由添加到临时黑色“指甲花纹身”中的对苯二胺（PPD）以及某些重金属引起的。
确定土耳其市售的商业临时黑色指甲花纹身混合物（n = 25）中是否存在并定量分析指甲花素、PPD 和重金属污染物（钴、镍、铅和铬）。
使用高效液相色谱法分析指甲花素和 PPD 的浓度，并通过电感耦合等离子体质谱法进行重金属定量分析。
在从纹身店购买的 25 个黑色指甲花纹身样本中均发现了 PPD；其含量在 3.37%至 51.6%之间不等。在所分析的 25 个临时黑色指甲花纹身样本中，有 21 个检测到了指甲花素（0.002-88.2%）。重金属污染物的浓度为 Co 为 0.44-3.11ppm，Ni 为 1.13-2.20ppm，Pb 为 1.59-17.7ppm，Cr 为 35.0-76.9ppm。
我们的研究结果表明，含有高达 51.6%PPD 水平的商业临时黑色指甲花混合物会对使用者造成接触致敏和严重过敏性接触性皮炎的风险。识别指甲花纹身产品的添加剂和金属污染物非常重要；金属污染物的意义仍有待评估。
